PDBsum entry 2r36

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protein metals Protein-protein interface(s) links
Hormone PDB id
Protein chains
22 a.a.
30 a.a. *
_NI ×4
Waters ×35
* Residue conservation analysis
PDB id:
Name: Hormone
Title: Crystal structure of ni human arg-insulin
Structure: Insulin. Chain: a, c. Fragment: insulin a chain. Insulin. Chain: b, d. Fragment: insulin b chain
Source: Homo sapiens. Human. Organism_taxid: 9606. Organism_taxid: 9606
2.00Å     R-factor:   0.225     R-free:   0.255
Authors: R.Sreekanth,V.Pattabhi,S.S.Rajan
Key ref: R.Sreekanth et al. (2009). Metal induced structural changes observed in hexameric insulin. Int J Biol Macromol, 44, 29-36. PubMed id: 18977386 DOI: 10.1016/j.ijbiomac.2008.09.019
29-Aug-07     Release date:   02-Sep-08    
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Protein chains
Pfam   ArchSchema ?
P01308  (INS_HUMAN) -  Insulin
110 a.a.
22 a.a.
Protein chains
Pfam   ArchSchema ?
P01308  (INS_HUMAN) -  Insulin
110 a.a.
30 a.a.
Key:    PfamA domain  Secondary structure

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   1 term 
  Biochemical function     hormone activity     1 term  


DOI no: 10.1016/j.ijbiomac.2008.09.019 Int J Biol Macromol 44:29-36 (2009)
PubMed id: 18977386  
Metal induced structural changes observed in hexameric insulin.
R.Sreekanth, V.Pattabhi, S.S.Rajan.
The metal ions in insulin hexamer play a crucial role in the T to R conformational transitions. We have determined the crystal structures of 2Mn2+, 1Rb1+ and 4Ni2+ human arg-insulin and compared them with the 2Zn2+ structure. The first two structures exist in the T3R3f state like the native 2Zn2+ arg-insulin, while the 4Ni2+ adopts a T6 conformation. The metal coordination is found to be tetrahedral in all the structures except that of nickel where a dual octahedral and tetrahedral coordination is found at one site. Rubidium occupies only one of the high affinity metal binding sites. The metal induced structural changes observed, have been explained.

Literature references that cite this PDB file's key reference

  PubMed id Reference
19834685 M.Kouach, B.Desbuquois, and F.Authier (2009).
Endosomal proteolysis of internalised [ArgA0]-human insulin at neutral pH generates the mature insulin peptide in rat liver in vivo.
  Diabetologia, 52, 2621-2632.  
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