PDBsum entry 2r2w

Hydrolase

PDB id: 2r2w

Contents

Protein chain

247 a.a. *

Ligands

SO4

4PG

Waters ×94

* Residue conservation analysis

PDB id:

2r2w

Name:

Hydrolase

Title:

Urokinase plasminogen activator b-chain-gppe complex

Structure:

Plasminogen activator, urokinase. Chain: u. Fragment: ligand binding domain. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: plau. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.01Å R-factor: 0.199 R-free: 0.233

Authors:

E.Zeslawska

Key ref:

E.Zesławska et al. (2007). Geometry of GPPE binding to picrate and to the urokinase type plasminogen activator. Bioorg Med Chem Lett, 17, 6212-6215. PubMed id: 17905583 DOI: 10.1016/j.bmcl.2007.09.020

Date:

28-Aug-07 Release date: 23-Oct-07

Protein chain

P00749  (UROK_HUMAN) -  Urokinase-type plasminogen activator from Homo sapiens

Seq: 431 a.a.

Struc: 247 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.4.21.73 - u-plasminogen activator.
• Reaction: Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.

DOI no: 10.1016/j.bmcl.2007.09.020

Bioorg Med Chem Lett 17:6212-6215 (2007)

PubMed id: 17905583

Geometry of GPPE binding to picrate and to the urokinase type plasminogen activator.

E.Zesławska, J.Stürzebecher, B.J.Oleksyn.

ABSTRACT

Crystal structure of 2-(4-guanidynephenyl)-1-phenyl-ethanone (GPPE) in two different environments was determined in order to compare the binding geometry of these compound to a simple picrate anion and to protein, urokinase-type plasminogen activator (uPA), which may be treated as a target for anti-cancer drugs. It was shown that the conformation and the hydrogen-bonding formation by GPPE molecule are similar in both environments, but several important differences were discovered and described.