spacer
spacer

PDBsum entry 2qzr

Go to PDB code: 
protein ligands metals links
Transferase PDB id
2qzr
Jmol
Contents
Protein chain
359 a.a. *
Ligands
S79
GOL ×2
Metals
_ZN
Waters ×187
* Residue conservation analysis
PDB id:
2qzr
Name: Transferase
Title: tRNA-guanine transglycosylase(tgt) in complex with 6-amino-2 naphthylmethyl)amino]-3,7-dihydro-8h-imidazo[4,5-g]quinazol
Structure: Queuine tRNA-ribosyltransferase. Chain: a. Synonym: tRNA-guanine transglycosylase, guanine insertion e engineered: yes
Source: Zymomonas mobilis. Organism_taxid: 542. Gene: tgt. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Resolution:
1.95Å     R-factor:   0.198     R-free:   0.259
Authors: S.R.Hoertner,T.Ritschel,B.Stengl,C.Kramer,W.B.Schweizer,B.Wa M.Kansy,G.Klebe,F.Diederich
Key ref: S.R.Hörtner et al. (2007). Potent inhibitors of tRNA-guanine transglycosylase, an enzyme linked to the pathogenicity of the Shigella bacterium: charge-assisted hydrogen bonding. Angew Chem Int Ed Engl, 46, 8266-8269. PubMed id: 17902085
Date:
17-Aug-07     Release date:   11-Sep-07    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P28720  (TGT_ZYMMO) -  Queuine tRNA-ribosyltransferase
Seq:
Struc:
386 a.a.
359 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.2.4.2.29  - tRNA-guanine(34) transglycosylase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction:
1. Guanine34 in tRNA + queuine = queuosine34 in tRNA + guanine
2. Guanine34 in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7- carbaguanine34 in tRNA + guanine
Guanine(34) in tRNA
+
queuine
Bound ligand (Het Group name = S79)
matches with 42.86% similarity
= queuosine(34) in tRNA
+ guanine
Guanine(34) in tRNA
+
7-aminomethyl-7-carbaguanine
Bound ligand (Het Group name = S79)
matches with 42.86% similarity
= 7-aminomethyl-7- carbaguanine(34) in tRNA
+ guanine
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     tRNA processing   3 terms 
  Biochemical function     transferase activity     4 terms  

 

 
    reference    
 
 
Angew Chem Int Ed Engl 46:8266-8269 (2007)
PubMed id: 17902085  
 
 
Potent inhibitors of tRNA-guanine transglycosylase, an enzyme linked to the pathogenicity of the Shigella bacterium: charge-assisted hydrogen bonding.
S.R.Hörtner, T.Ritschel, B.Stengl, C.Kramer, W.B.Schweizer, B.Wagner, M.Kansy, G.Klebe, F.Diederich.
 
  ABSTRACT  
 
No abstract given.