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PDBsum entry 2o23

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protein ligands Protein-protein interface(s) links
Oxidoreductase PDB id
2o23
Jmol
Contents
Protein chains
248 a.a. *
Ligands
NAD ×2
GOL
Waters ×621
* Residue conservation analysis
PDB id:
2o23
Name: Oxidoreductase
Title: The structure of wild-type human hadh2 (17beta-hydroxysteroi dehydrogenase type 10) bound to NAD+ at 1.2 a
Structure: Hadh2 protein. Chain: a, b. Synonym: hydroxyacyl-coenzyme a dehydrogenase, type ii. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: hadh2. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Biol. unit: Tetramer (from PDB file)
Resolution:
1.20Å     R-factor:   0.123     R-free:   0.154
Authors: K.L.Kavanagh,N.Shafqat,K.Scholer,J.Debreczeni,J.Zschocke,F.V J.Weigelt,C.Arrowsmith,M.Sundstrom,A.Edwards,U.Oppermann,St Genomics Consortium (Sgc)
Key ref: K.L.Kavanagh et al. The structure of wild-Type human hadh2 (17beta-Hydroxysteroid dehydrogenase type 10) bound to NAD+ at 1.2 a. To be published, .
Date:
29-Nov-06     Release date:   12-Dec-06    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q99714  (HCD2_HUMAN) -  3-hydroxyacyl-CoA dehydrogenase type-2
Seq:
Struc:
261 a.a.
248 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class 2: E.C.1.1.1.178  - 3-hydroxy-2-methylbutyryl-CoA dehydrogenase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: (2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD+ = 2-methylacetoacetyl-CoA + NADH
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA
+
NAD(+)
Bound ligand (Het Group name = NAD)
corresponds exactly
= 2-methylacetoacetyl-CoA
+ NADH
   Enzyme class 3: E.C.1.1.1.35  - 3-hydroxyacyl-CoA dehydrogenase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH
(S)-3-hydroxyacyl-CoA
+
NAD(+)
Bound ligand (Het Group name = NAD)
corresponds exactly
= 3-oxoacyl-CoA
+ NADH
   Enzyme class 4: E.C.1.1.1.51  - 3(or 17)-beta-hydroxysteroid dehydrogenase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Testosterone + NAD(P)(+) = androst-4-ene-3,17-dione + NAD(P)H
Testosterone
+ NAD(P)(+)
= androst-4-ene-3,17-dione
+ NAD(P)H
Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     plasma membrane   6 terms 
  Biological process     metabolic process   7 terms 
  Biochemical function     protein binding     7 terms