PDBsum entry 2nyq

Hydrolase

PDB id: 2nyq

Contents

Protein chain

291 a.a. *

Ligands

TRP

Metals

_ZN ×2

Waters ×152

* Residue conservation analysis

PDB id:

2nyq

Name:

Hydrolase

Title:

Structure of vibrio proteolyticus aminopeptidase with a bound trp fragment of dlwcf

Structure:

Aminopeptidase. Chain: a. Fragment: bacterial leucyl aminopeptidase, residues 97-405. Tetrapeptide. Chain: b. Engineered: yes. Other_details: d-leu-l-trp-l-cys-l-phe

Source:

Vibrio proteolyticus. Organism_taxid: 671. Strain: attc 15338, dsm 30189, ncbm 1326. Synthetic: yes. Synthetic construct. Organism_taxid: 32630. Other_details: the tetrapeptide was chemically synthesised using solid-phase manual synthesis via fmoc-derivatized amino acids, on rink-amide resin.

Resolution:

2.50Å R-factor: 0.191 R-free: 0.256

Authors:

B.Bennett,A.Kumar,B.Narayanan,J.-J.Kim

Key ref:

A.Kumar et al. Substrate recognition by the leucine aminopeptidase from vibrio proteolyticus. To be published, .

Date:

21-Nov-06 Release date: 30-Oct-07

Protein chain

Q01693  (AMPX_VIBPR) -  Bacterial leucyl aminopeptidase from Vibrio proteolyticus

Seq: 504 a.a.

Struc: 291 a.a.

Enzyme reactions

• Enzyme class: E.C.3.4.11.10 - bacterial leucyl aminopeptidase.
• Reaction: Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
• Cofactor: Zn(2+)