PDBsum entry 2jv1

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protein Protein-protein interface(s) links
Hormone PDB id
Protein chains
21 a.a.
30 a.a. *
* Residue conservation analysis
PDB id:
Name: Hormone
Title: Nmr structure of human insulin monomer in 35% cd3cn zinc free, 50 structures
Structure: Insulin. Chain: a. Fragment: insulin a chain: residues 90-110. Engineered: yes. Insulin. Chain: b. Fragment: insulin b chain: residues 25-54. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: ins. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562
NMR struc: 50 models
Authors: W.Bocian,L.Kozerski
Key ref: W.Bocian et al. (2008). Structure of human insulin monomer in water/acetonitrile solution. J Biomol NMR, 40, 55-64. PubMed id: 18040865
11-Sep-07     Release date:   11-Dec-07    
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Protein chain
Pfam   ArchSchema ?
P01308  (INS_HUMAN) -  Insulin
110 a.a.
21 a.a.
Protein chain
Pfam   ArchSchema ?
P01308  (INS_HUMAN) -  Insulin
110 a.a.
30 a.a.
Key:    PfamA domain  Secondary structure

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   1 term 
  Biochemical function     hormone activity     1 term  


J Biomol NMR 40:55-64 (2008)
PubMed id: 18040865  
Structure of human insulin monomer in water/acetonitrile solution.
W.Bocian, J.Sitkowski, E.Bednarek, A.Tarnowska, R.Kawecki, L.Kozerski.
Here we present evidence that in water/acetonitrile solvent detailed structural and dynamic information can be obtained for important proteins that are naturally present as oligomers under native conditions. An NMR-derived human insulin monomer structure in H2O/CD3CN, 65/35 vol%, pH 3.6 is presented and compared with the available X-ray structure of a monomer that forms part of a hexamer (Acta Crystallogr. 2003 Sec. D59, 474) and with NMR structures in water and organic cosolvent. Detailed analysis using PFGSE NMR, temperature-dependent NMR, dilution experiments and CSI proves that the structure is monomeric in the concentration and temperature ranges 0.1-3 mM and 10-30 degrees C, respectively. The presence of long-range interstrand NOEs, as found in the crystal structure of the monomer, provides the evidence for conservation of the tertiary structure. Starting from structures calculated by the program CYANA, two different molecular dynamics simulated annealing refinement protocols were applied, either using the program AMBER in vacuum (AMBER_VC), or including a generalized Born solvent model (AMBER_GB).

Literature references that cite this PDB file's key reference

  PubMed id Reference
20697659 A.C.Welinder, J.Zhang, D.B.Steensgaard, and J.Ulstrup (2010).
Adsorption of human insulin on single-crystal gold surfaces investigated by in situ scanning tunnelling microscopy and electrochemistry.
  Phys Chem Chem Phys, 12, 9999.  
20632994 A.Noormägi, J.Gavrilova, J.Smirnova, V.Tõugu, and P.Palumaa (2010).
Zn(II) ions co-secreted with insulin suppress inherent amyloidogenic properties of monomeric insulin.
  Biochem J, 430, 511-518.  
20133841 J.Jirácek, L.Záková, E.Antolíková, C.J.Watson, J.P.Turkenburg, G.G.Dodson, and A.M.Brzozowski (2010).
Implications for the active form of human insulin based on the structural convergence of highly active hormone analogues.
  Proc Natl Acad Sci U S A, 107, 1966-1970.
PDB codes: 2wrv 2wrw 2wrx 2ws0 2ws1 2ws4 2ws6 2ws7
20336256 Z.Ganim, K.C.Jones, and A.Tokmakoff (2010).
Insulin dimer dissociation and unfolding revealed by amide I two-dimensional infrared spectroscopy.
  Phys Chem Chem Phys, 12, 3579-3588.  
19086273 E.E.Büllesbach, M.A.Hass, M.R.Jensen, D.F.Hansen, S.M.Kristensen, C.Schwabe, and J.J.Led (2008).
Solution structure of a conformationally restricted fully active derivative of the human relaxin-like factor.
  Biochemistry, 47, 13308-13317.
PDB codes: 2k6t 2k6u
18260111 W.Bocian, J.Sitkowski, A.Tarnowska, E.Bednarek, R.Kawecki, W.Koźmiński, and L.Kozerski (2008).
Direct insight into insulin aggregation by 2D NMR complemented by PFGSE NMR.
  Proteins, 71, 1057-1065.  
18491415 W.Bocian, P.Borowicz, J.Mikołajczyk, J.Sitkowski, A.Tarnowska, E.Bednarek, T.Głabski, B.Tejchman-Małecka, M.Bogiel, and L.Kozerski (2008).
NMR structure of biosynthetic engineered human insulin monomer B31(Lys)-B32(Arg) in water/acetonitrile solution. Comparison with the solution structure of native human insulin monomer.
  Biopolymers, 89, 820-830.
PDB code: 2rn5
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