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PDBsum entry 2j9n
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protein ligands metals Protein-protein interface(s) links
Hydrolase PDB id
2j9n

 

 

 

 

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Contents
Protein chains
223 a.a. *
15 a.a. *
Ligands
GLN-UNK-UNK
BEN
IPH
Metals
_CA
_NA
Waters ×208
* Residue conservation analysis
PDB id:
2j9n
Name: Hydrolase
Title: Robotically harvested trypsin complexed with benzamidine containing polypeptide mediated crystal contacts
Structure: Cationic trypsin. Chain: a. Fragment: residues 21-243. Unknown peptide. Chain: b. Engineered: yes. Unknown peptide. Chain: c. Engineered: yes
Source: Bos taurus. Bovine. Organism_taxid: 9913. Organ: pancreas. Tissue: smooth. Other_details: sigma. Synthetic: yes. Synthetic construct. Organism_taxid: 32630.
Resolution:
1.50Å     R-factor:   0.155     R-free:   0.176
Authors: R.Viola,P.Carman,J.Walsh,E.Miller,M.Benning,D.Frankel,A.Mcpherson, R.Cudney,B.Rupp
Key ref: R.Viola et al. (2007). Operator-assisted harvesting of protein crystals using a universal micromanipulation robot. J Appl Crystallogr, 40, 539-545. PubMed id: 19461845
Date:
14-Nov-06     Release date:   03-Apr-07    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P00760  (TRY1_BOVIN) -  Serine protease 1 from Bos taurus
Seq:
Struc: 		246 a.a.
223 a.a.
Protein chain
No UniProt id for this chain
Struc: 15 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: Chain A: E.C.3.4.21.4  - trypsin.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

 

 
J Appl Crystallogr 40:539-545 (2007)
PubMed id: 19461845  
 
 
Operator-assisted harvesting of protein crystals using a universal micromanipulation robot.
R.Viola, P.Carman, J.Walsh, E.Miller, M.Benning, D.Frankel, A.McPherson, B.Cudney, B.Rupp.
 
  ABSTRACT  
 
High-throughput crystallography has reached a level of automation where complete computer-assisted robotic crystallization pipelines are capable of cocktail preparation, crystallization plate setup, and inspection and interpretation of results. While mounting of crystal pins, data collection and structure solution are highly automated, crystal harvesting and cryocooling remain formidable challenges towards full automation. To address the final frontier in achieving fully automated high-throughput crystallography, the prototype of an anthropomorphic six-axis universal micromanipulation robot (UMR) has been designed and tested; this UMR is capable of operator-assisted harvesting and cryoquenching of protein crystals as small as 10 microm from a variety of 96-well plates. The UMR is equipped with a versatile tool exchanger providing full operational flexibility. Trypsin crystals harvested and cryoquenched using the UMR have yielded a 1.5 A structure demonstrating the feasibility of robotic protein crystal harvesting.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20526815 M.Cymborowski, M.Klimecka, M.Chruszcz, M.D.Zimmerman, I.A.Shumilin, D.Borek, K.Lazarski, A.Joachimiak, Z.Otwinowski, W.Anderson, and W.Minor (2010).
To automate or not to automate: this is the question.
  J Struct Funct Genomics, 11, 211-221.  
  18097093 T.S.Walter, E.J.Mancini, J.Kadlec, S.C.Graham, R.Assenberg, J.Ren, S.Sainsbury, R.J.Owens, D.I.Stuart, J.M.Grimes, and K.Harlos (2008).
Semi-automated microseeding of nanolitre crystallization experiments.
  Acta Crystallogr Sect F Struct Biol Cryst Commun, 64, 14-18.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.

 

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