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PDBsum entry 2i9p

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protein ligands Protein-protein interface(s) links
Oxidoreductase PDB id
2i9p
Jmol
Contents
Protein chain
293 a.a. *
Ligands
NAD ×4
* Residue conservation analysis
PDB id:
2i9p
Name: Oxidoreductase
Title: Crystal structure of human hydroxyisobutyrate dehydrogenase with NAD+
Structure: 3-hydroxyisobutyrate dehydrogenase. Chain: a, b, c, d. Synonym: hibadh. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: hibadh. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Biol. unit: Tetramer (from PQS)
Resolution:
2.55Å     R-factor:   0.176     R-free:   0.220
Authors: K.L.Kavanagh,E.Papagrigoriou,E.Salah,P.Lukacik,C.Smee,N.Burg Delft,J.Weigelt,C.Arrowsmith,M.Sundstrom,A.Edwards,U.Opperm Structural Genomics Consortium (Sgc)
Key ref: K.L.Kavanagh et al. Crystal structure of human hydroxyisobutyrate dehydrogenase complexed with NAD+. To be published, .
Date:
06-Sep-06     Release date:   26-Sep-06    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P31937  (3HIDH_HUMAN) -  3-hydroxyisobutyrate dehydrogenase, mitochondrial
Seq:
Struc:
336 a.a.
293 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.1.1.1.31  - 3-hydroxyisobutyrate dehydrogenase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH
3-hydroxy-2-methylpropanoate
+
NAD(+)
Bound ligand (Het Group name = NAD)
corresponds exactly
= 2-methyl-3-oxopropanoate
+ NADH
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     oxidation-reduction process   3 terms 
  Biochemical function     oxidoreductase activity     6 terms