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PDBsum entry 2g5w

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protein ligands Protein-protein interface(s) links
Oxidoreductase PDB id
2g5w
Jmol PyMol
Contents
Protein chains
363 a.a. *
Ligands
FMN ×2
8PG ×2
Waters ×108
* Residue conservation analysis
PDB id:
2g5w
Name: Oxidoreductase
Title: X-ray crystal structure of arabidopsis thaliana 12-oxophytod reductase isoform 3 (atopr3) in complex with 8-iso prostagl and its cofactor, flavin mononucleotide.
Structure: 12-oxophytodienoate reductase 3. Chain: a, b. Synonym: 12-oxophytodienoate- 10,11-reductase 3, opda-reduc delayed dehiscence 1, atopr3. Engineered: yes
Source: Arabidopsis thaliana. Thale cress. Organism_taxid: 3702. Gene: at2g06050. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
2.58Å     R-factor:   0.205     R-free:   0.264
Authors: B.W.Han,T.E.Malone,C.A.Bingman,G.E.Wesenberg,G.N.Phillips Jr B.G.Fox,Center For Eukaryotic Structural Genomics (Cesg)
Key ref: B.W.Han et al. (2011). Crystal structure of Arabidopsis thaliana 12-oxophytodienoate reductase isoform 3 in complex with 8-iso prostaglandin A(1). Proteins, 79, 3236-3241. PubMed id: 21915915
Date:
23-Feb-06     Release date:   04-Apr-06    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q9FUP0  (OPR3_ARATH) -  12-oxophytodienoate reductase 3
Seq:
Struc:
391 a.a.
363 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.1.3.1.42  - 12-oxophytodienoate reductase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: 8-((1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl)octanoate + NADP+ = (15Z)-12-oxophyto-10,15-dienoate + NADPH
8-((1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl)octanoate
+ NADP(+)
=
(15Z)-12-oxophyto-10,15-dienoate
Bound ligand (Het Group name = 8PG)
matches with 87.50% similarity
+ NADPH
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     peroxisome   1 term 
  Biological process     oxidation-reduction process   9 terms 
  Biochemical function     catalytic activity     4 terms  

 

 
    reference    
 
 
Proteins 79:3236-3241 (2011)
PubMed id: 21915915  
 
 
Crystal structure of Arabidopsis thaliana 12-oxophytodienoate reductase isoform 3 in complex with 8-iso prostaglandin A(1).
B.W.Han, T.E.Malone, D.J.Kim, C.A.Bingman, H.J.Kim, B.G.Fox, G.N.Phillips.
 
  ABSTRACT  
 
No abstract given.

 

 

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