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PDBsum entry 2f4w

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protein Protein-protein interface(s) links
Ligase PDB id
2f4w
Jmol
Contents
Protein chains
147 a.a. *
Waters ×245
* Residue conservation analysis
PDB id:
2f4w
Name: Ligase
Title: Human ubiquitin-conjugating enzyme e2 j2
Structure: Ubiquitin-conjugating enzyme e2, j2. Chain: a, b. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: ube2j2. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.
Resolution:
2.00Å     R-factor:   0.190     R-free:   0.250
Authors: J.R.Walker,G.V.Avvakumov,S.Xue,P.J.Finerty Jr.,E.M.Newman, F.Mackenzie,J.Weigelt,M.Sundstrom,C.Arrowsmith,A.Edwards, A.Bochkarev,S.Dhe-Paganon,Structural Genomics Consortium (S
Key ref: Y.Sheng et al. (2012). A human ubiquitin conjugating enzyme (E2)-HECT E3 ligase structure-function screen. Mol Cell Proteomics, 11, 329-341. PubMed id: 22496338
Date:
24-Nov-05     Release date:   27-Dec-05    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q8N2K1  (UB2J2_HUMAN) -  Ubiquitin-conjugating enzyme E2 J2
Seq:
Struc:
259 a.a.
147 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.6.3.2.19  - Ubiquitin--protein ligase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine
ATP
+ ubiquitin
+ protein lysine
= AMP
+ diphosphate
+ protein N-ubiquityllysine
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 
    reference    
 
 
Mol Cell Proteomics 11:329-341 (2012)
PubMed id: 22496338  
 
 
A human ubiquitin conjugating enzyme (E2)-HECT E3 ligase structure-function screen.
Y.Sheng, J.H.Hong, R.Doherty, T.Srikumar, J.Shloush, G.V.Avvakumov, J.R.Walker, S.Xue, D.Neculai, J.W.Wan, S.K.Kim, C.H.Arrowsmith, B.Raught, S.Dhe-Paganon.
 
  ABSTRACT  
 
No abstract given.