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PDBsum entry 2cv1

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protein dna_rna ligands metals links
Ligase/RNA PDB id
2cv1
Jmol
Contents
Protein chains
468 a.a. *
DNA/RNA
Ligands
ATP ×2
GAU ×2
Metals
_MG ×4
Waters ×182
* Residue conservation analysis
PDB id:
2cv1
Name: Ligase/RNA
Title: Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu), atp, and an analog of l-glutamate: a quaternary complex
Structure: tRNA. Chain: c, d. Engineered: yes. Glutamyl-tRNA synthetase. Chain: a, b. Synonym: glutamate--tRNA ligase, glurs. Engineered: yes
Source: Synthetic: yes. Other_details: in vitro transcription. Thermus thermophilus. Organism_taxid: 274. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Biol. unit: Dimer (from PQS)
Resolution:
2.41Å     R-factor:   0.221     R-free:   0.277
Authors: S.Sekine,S.Yokoyama,Riken Structural Genomics/proteomics Initiative (Rsgi)
Key ref: S.Sekine and s.yokoyama Glutamyl-Trna synthetase from thermus thermophilus in complex with tRNA(glu), Atp, And an analog of l-Glutamate: a quaternary complex. To be published, .
Date:
31-May-05     Release date:   05-Sep-06    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P27000  (SYE_THET8) -  Glutamate--tRNA ligase
Seq:
Struc:
468 a.a.
468 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.6.1.1.17  - Glutamate--tRNA ligase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)
ATP
Bound ligand (Het Group name = ATP)
corresponds exactly
+
L-glutamate
Bound ligand (Het Group name = GAU)
matches with 90.00% similarity
+ tRNA(Glu)
= AMP
+ diphosphate
+ L-glutamyl-tRNA(Glu)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     cytoplasm   1 term 
  Biological process     translation   4 terms 
  Biochemical function     nucleotide binding     7 terms