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PDBsum entry 2cbx

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protein ligands Protein-protein interface(s) links
Transferase PDB id
2cbx
Jmol
Contents
Protein chains
291 a.a. *
Ligands
CC5 ×3
GOL ×9
Waters ×495
* Residue conservation analysis
PDB id:
2cbx
Name: Transferase
Title: X-ray crystal structure of 5'-fluorodeoxyadenosine synthase from streptomyces cattleya complexed with beta-d- erythrofuranosyl-adenosine
Structure: 5'-fluoro-5'-deoxyadenosine synthase. Chain: a, b, c. Engineered: yes
Source: Streptomyces cattleya. Organism_taxid: 29303. Expressed in: escherichia coli. Expression_system_taxid: 511693. Expression_system_variant: de3.
Resolution:
2.00Å     R-factor:   0.176     R-free:   0.239
Authors: A.R.Mcewan,C.D.Cadicamo,H.Deng,R.P.Mcglinchey,D.R.Robinson, D.O'Hagan,J.H.Naismith,J.Spencer
Key ref: S.L.Cobb et al. (2006). Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates. Org Biomol Chem, 4, 1458-1460. PubMed id: 16604208
Date:
09-Jan-06     Release date:   06-Mar-07    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q70GK9  (Q70GK9_STRCT) -  5'-fluoro-5'-deoxy-adenosine synthase
Seq:
Struc:
299 a.a.
291 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 

 
Org Biomol Chem 4:1458-1460 (2006)
PubMed id: 16604208  
 
 
Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates.
S.L.Cobb, H.Deng, A.R.McEwan, J.H.Naismith, D.O'Hagan, D.A.Robinson.
 
  ABSTRACT  
 
The fluorinase enzyme from Streptomyces cattleya displays an unusual ability in biocatalysis in that it forms a C-F bond. We now report that the enzyme will accept 2'-deoxyadenosine in place of adenosine substrates, and structural evidence reveals a reorganisation in hydrogen bonding to accommodate this substrate series. It emerges from this study that the enzyme does not require a planar ribose conformation of the substrate to catalyse C-F bond formation.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20354314 P.C.Kline, H.Zhao, B.C.Noll, A.G.Oliver, and A.S.Serianni (2010).
1-(beta-D-Erythrofuranosyl)adenosine.
  Acta Crystallogr C, 66, o215-o218.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.