PDBsum entry 2c2w

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protein ligands metals Protein-protein interface(s) links
Transferase PDB id
Protein chains
291 a.a. *
5CD ×3
_CL ×7
Waters ×559
* Residue conservation analysis
PDB id:
Name: Transferase
Title: The fluorinase from streptomyces cattleya is also a chlorinase. Structure of 5'-chloro-5'-deoxyadenosine crystallised in the fluorinase.
Structure: 5'-fluoro-5'-deoxyadenosine synthase. Chain: a, b, c. Engineered: yes
Source: Streptomyces cattleya. Organism_taxid: 29303. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Biol. unit: Hexamer (from PDB file)
2.00Å     R-factor:   0.198     R-free:   0.249
Authors: A.R.Mcewan,J.H.Naismith,D.O'Hagan
Key ref: H.Deng et al. (2006). The fluorinase from Streptomyces cattleya is also a chlorinase. Angew Chem Int Ed Engl, 45, 759-762. PubMed id: 16370017
30-Sep-05     Release date:   19-Dec-05    
Go to PROCHECK summary

Protein chains
Pfam   ArchSchema ?
Q70GK9  (Q70GK9_STRCT) -  5'-fluoro-5'-deoxy-adenosine synthase
299 a.a.
291 a.a.
Key:    PfamA domain  Secondary structure  CATH domain


Angew Chem Int Ed Engl 45:759-762 (2006)
PubMed id: 16370017  
The fluorinase from Streptomyces cattleya is also a chlorinase.
H.Deng, S.L.Cobb, A.R.McEwan, R.P.McGlinchey, J.H.Naismith, D.O'Hagan, D.A.Robinson, J.B.Spencer.


Literature references that cite this PDB file's key reference

  PubMed id Reference
20085308 A.S.Eustáquio, D.O'Hagan, and B.S.Moore (2010).
Engineering fluorometabolite production: fluorinase expression in Salinispora tropica Yields Fluorosalinosporamide.
  J Nat Prod, 73, 378-382.  
19675645 A.Butler, and M.Sandy (2009).
Mechanistic considerations of halogenating enzymes.
  Nature, 460, 848-854.  
19841722 J.R.Sufrin, S.Finckbeiner, and C.M.Oliver (2009).
Marine-derived metabolites of S-adenosylmethionine as templates for new anti-infectives.
  Mar Drugs, 7, 401-434.  
18059261 A.S.Eustáquio, F.Pojer, J.P.Noel, and B.S.Moore (2008).
Discovery and characterization of a marine bacterial SAM-dependent chlorinase.
  Nat Chem Biol, 4, 69-74.
PDB codes: 2q6i 2q6k 2q6l 2q6o
18720493 A.S.Eustáquio, J.Härle, J.P.Noel, and B.S.Moore (2008).
S-Adenosyl-L-methionine hydrolase (adenosine-forming), a conserved bacterial and archaeal protein related to SAM-dependent halogenases.
  Chembiochem, 9, 2215-2219.  
18675376 H.Deng, and D.O'Hagan (2008).
The fluorinase, the chlorinase and the duf-62 enzymes.
  Curr Opin Chem Biol, 12, 582-592.  
17910070 K.N.Rao, S.K.Burley, and S.Swaminathan (2008).
Crystal structure of a conserved protein of unknown function (MJ1651) from Methanococcus jannaschii.
  Proteins, 70, 572-577.
PDB code: 2f4n
18818720 R.Mazumder, and S.Vasudevan (2008).
Structure-guided comparative analysis of proteins: principles, tools, and applications for predicting function.
  PLoS Comput Biol, 4, e1000151.  
18330472 X.Chen, and K.H.van Pée (2008).
Catalytic mechanisms, basic roles, and biotechnological and environmental significance of halogenating enzymes.
  Acta Biochim Biophys Sin (Shanghai), 40, 183-193.  
17881282 D.G.Fujimori, and C.T.Walsh (2007).
What's new in enzymatic halogenations.
  Curr Opin Chem Biol, 11, 553-560.  
17898899 P.H.Buist (2007).
Exotic biomodification of fatty acids.
  Nat Prod Rep, 24, 1110-1127.  
16604208 S.L.Cobb, H.Deng, A.R.McEwan, J.H.Naismith, D.O'Hagan, and D.A.Robinson (2006).
Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates.
  Org Biomol Chem, 4, 1458-1460.
PDB codes: 2c4u 2c5b 2cbx 2cc2
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