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PDBsum entry 2c2w

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protein ligands metals Protein-protein interface(s) links
Transferase PDB id
2c2w
Jmol
Contents
Protein chains
291 a.a. *
Ligands
5CD ×3
Metals
_CL ×7
Waters ×559
* Residue conservation analysis
PDB id:
2c2w
Name: Transferase
Title: The fluorinase from streptomyces cattleya is also a chlorinase. Structure of 5'-chloro-5'-deoxyadenosine crystallised in the fluorinase.
Structure: 5'-fluoro-5'-deoxyadenosine synthase. Chain: a, b, c. Engineered: yes
Source: Streptomyces cattleya. Organism_taxid: 29303. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Biol. unit: Hexamer (from PDB file)
Resolution:
2.00Å     R-factor:   0.198     R-free:   0.249
Authors: A.R.Mcewan,J.H.Naismith,D.O'Hagan
Key ref: H.Deng et al. (2006). The fluorinase from Streptomyces cattleya is also a chlorinase. Angew Chem Int Ed Engl, 45, 759-762. PubMed id: 16370017
Date:
30-Sep-05     Release date:   19-Dec-05    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q70GK9  (Q70GK9_STRCT) -  5'-fluoro-5'-deoxy-adenosine synthase
Seq:
Struc:
299 a.a.
291 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 

 
Angew Chem Int Ed Engl 45:759-762 (2006)
PubMed id: 16370017  
 
 
The fluorinase from Streptomyces cattleya is also a chlorinase.
H.Deng, S.L.Cobb, A.R.McEwan, R.P.McGlinchey, J.H.Naismith, D.O'Hagan, D.A.Robinson, J.B.Spencer.
 
  ABSTRACT  
 

 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20085308 A.S.Eustáquio, D.O'Hagan, and B.S.Moore (2010).
Engineering fluorometabolite production: fluorinase expression in Salinispora tropica Yields Fluorosalinosporamide.
  J Nat Prod, 73, 378-382.  
19675645 A.Butler, and M.Sandy (2009).
Mechanistic considerations of halogenating enzymes.
  Nature, 460, 848-854.  
19841722 J.R.Sufrin, S.Finckbeiner, and C.M.Oliver (2009).
Marine-derived metabolites of S-adenosylmethionine as templates for new anti-infectives.
  Mar Drugs, 7, 401-434.  
18059261 A.S.Eustáquio, F.Pojer, J.P.Noel, and B.S.Moore (2008).
Discovery and characterization of a marine bacterial SAM-dependent chlorinase.
  Nat Chem Biol, 4, 69-74.
PDB codes: 2q6i 2q6k 2q6l 2q6o
18720493 A.S.Eustáquio, J.Härle, J.P.Noel, and B.S.Moore (2008).
S-Adenosyl-L-methionine hydrolase (adenosine-forming), a conserved bacterial and archaeal protein related to SAM-dependent halogenases.
  Chembiochem, 9, 2215-2219.  
18675376 H.Deng, and D.O'Hagan (2008).
The fluorinase, the chlorinase and the duf-62 enzymes.
  Curr Opin Chem Biol, 12, 582-592.  
17910070 K.N.Rao, S.K.Burley, and S.Swaminathan (2008).
Crystal structure of a conserved protein of unknown function (MJ1651) from Methanococcus jannaschii.
  Proteins, 70, 572-577.
PDB code: 2f4n
18818720 R.Mazumder, and S.Vasudevan (2008).
Structure-guided comparative analysis of proteins: principles, tools, and applications for predicting function.
  PLoS Comput Biol, 4, e1000151.  
18330472 X.Chen, and K.H.van Pée (2008).
Catalytic mechanisms, basic roles, and biotechnological and environmental significance of halogenating enzymes.
  Acta Biochim Biophys Sin (Shanghai), 40, 183-193.  
17881282 D.G.Fujimori, and C.T.Walsh (2007).
What's new in enzymatic halogenations.
  Curr Opin Chem Biol, 11, 553-560.  
17898899 P.H.Buist (2007).
Exotic biomodification of fatty acids.
  Nat Prod Rep, 24, 1110-1127.  
16604208 S.L.Cobb, H.Deng, A.R.McEwan, J.H.Naismith, D.O'Hagan, and D.A.Robinson (2006).
Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates.
  Org Biomol Chem, 4, 1458-1460.
PDB codes: 2c4u 2c5b 2cbx 2cc2
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.