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PDBsum entry 2bsp

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protein metals links
Lyase PDB id
2bsp
Jmol
Contents
Protein chain
399 a.a. *
Metals
_CA
Waters ×246
* Residue conservation analysis
PDB id:
2bsp
Name: Lyase
Title: Bacillus subtilis pectate lyase r279k mutant
Structure: Protein (pectate lyase). Chain: a. Engineered: yes. Mutation: yes
Source: Bacillus subtilis. Organism_taxid: 1423. Strain: s1103. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Resolution:
1.80Å     R-factor:   0.196     R-free:   0.225
Authors: R.Pickersgill
Key ref: R.Pickersgill et al. The conserved arginine proximal to the essential calcium of bacillus subtilis pectate lyase stabilizes the transition state. To be published, .
Date:
31-Jul-98     Release date:   12-Aug-98    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P39116  (PLY_BACSU) -  Pectate lyase
Seq:
Struc:
420 a.a.
399 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.4.2.2.2  - Pectate lyase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway:
Pectin and Pectate Lyases
      Reaction: Eliminative cleavage of pectate to give oligosaccharides with 4-deoxy- alpha-D-gluc-4-enuronosyl groups at their non-reducing ends.