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PDBsum entry 2ax4

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protein ligands Protein-protein interface(s) links
Transferase PDB id
2ax4
Jmol
Contents
Protein chains
198 a.a. *
Ligands
ADP ×4
Waters ×292
* Residue conservation analysis
PDB id:
2ax4
Name: Transferase
Title: Crystal structure of the kinase domain of human 3'- phosphoadenosine 5'-phosphosulphate synthetase 2
Structure: Bifunctional 3'-phosphoadenosine 5'- phosphosulfate synthethase 2. Chain: a, b, c, d. Fragment: aps kinase domain (residues 21-218). Synonym: adenylylsulfate 3'-phosphotransferase, adenosine- 5'-phosphosulfate 3'-phosphotransferase, 3'- phosphoadenosine-5'-phosphosulfate synthetase. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: papss2. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.
Biol. unit: Dimer (from PQS)
Resolution:
2.50Å     R-factor:   0.215     R-free:   0.278
Authors: W.M.Rabeh,L.Nedyalkova,S.Ismail,H.Park,C.Arrowsmith, A.Edwards,M.Sundstrom,J.Weigelt,A.Bochkarev,Structural Genomics Consortium (Sgc)
Key ref: W.M.Rabeh et al. Crystal structure of the kinase domain of papss 2. To be published, .
Date:
02-Sep-05     Release date:   20-Sep-05    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
O95340  (PAPS2_HUMAN) -  Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2
Seq:
Struc:
 
Seq:
Struc:
614 a.a.
198 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class 2: E.C.2.7.1.25  - Adenylyl-sulfate kinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate
ATP
+ adenylyl sulfate
=
ADP
Bound ligand (Het Group name = ADP)
corresponds exactly
+ 3'-phosphoadenylyl sulfate
   Enzyme class 3: E.C.2.7.7.4  - Sulfate adenylyltransferase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + sulfate = diphosphate + adenylyl sulfate
ATP
Bound ligand (Het Group name = ADP)
matches with 87.00% similarity
+ sulfate
= diphosphate
+ adenylyl sulfate
Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     sulfate assimilation   1 term 
  Biochemical function     adenylylsulfate kinase activity     2 terms