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PDBsum entry 2awf

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protein links
Ligase PDB id
2awf
Jmol
Contents
Protein chain
122 a.a. *
Waters ×53
* Residue conservation analysis
PDB id:
2awf
Name: Ligase
Title: Structure of human ubiquitin-conjugating enzyme e2 g1
Structure: Ubiquitin-conjugating enzyme e2 g1. Chain: a. Fragment: catalytic (ubcc) domain, residues 7-159. Synonym: ubiquitin-protein ligase g1, ubiquitin carrier pro e217k, ubc7. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: ube2g1, ube2g. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Biol. unit: Dimer (from PQS)
Resolution:
2.10Å     R-factor:   0.223     R-free:   0.263
Authors: J.R.Walker,G.V.Avvakumov,S.Xue,E.M.Newman,P.Finerty,F.Macken J.Weigelt,M.Sundstrom,C.Arrowsmith,A.Edwards,A.Bochkarev,S. Paganon,Structural Genomics Consortium (Sgc)
Key ref: Y.Sheng et al. (2012). A human ubiquitin conjugating enzyme (E2)-HECT E3 ligase structure-function screen. Mol Cell Proteomics, 11, 329-341. PubMed id: 22496338 DOI: 10.1074/mcp.O111.013706
Date:
01-Sep-05     Release date:   20-Sep-05    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P62253  (UB2G1_HUMAN) -  Ubiquitin-conjugating enzyme E2 G1
Seq:
Struc:
170 a.a.
122 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 7 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.6.3.2.19  - Ubiquitin--protein ligase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine
ATP
+ ubiquitin
+ protein lysine
= AMP
+ diphosphate
+ protein N-ubiquityllysine
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular vesicular exosome   1 term 
  Biological process     protein K48-linked ubiquitination   4 terms 
  Biochemical function     nucleotide binding     6 terms  

 

 
    reference    
 
 
DOI no: 10.1074/mcp.O111.013706 Mol Cell Proteomics 11:329-341 (2012)
PubMed id: 22496338  
 
 
A human ubiquitin conjugating enzyme (E2)-HECT E3 ligase structure-function screen.
Y.Sheng, J.H.Hong, R.Doherty, T.Srikumar, J.Shloush, G.V.Avvakumov, J.R.Walker, S.Xue, D.Neculai, J.W.Wan, S.K.Kim, C.H.Arrowsmith, B.Raught, S.Dhe-Paganon.
 
  ABSTRACT  
 
No abstract given.