The crystal structure of putative precorrin isomerase CbiC in cobalamin biosynthesis.
The leptospira cbiC encodes the enzyme catalyzing the methyl rearrangement
reaction of the cobalamin biosynthesis pathway. The protein has been cloned and
overexpressed as a His-tagged recombinant protein in Escherichia coli. The
crystal structures have been solved in two crystal forms (P4(2)2(1)2 and
P3(1)21) diffracting to 3.0 and 2.3A resolution, respectively. The structures
are similar to the precorrin-8x methyl mutase (CobH), an enzyme of the aerobic
pathway to vitamin B12.