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PDBsum entry 2afr

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protein links
Isomerase PDB id
2afr
Jmol PyMol
Contents
Protein chain
216 a.a. *
Waters ×75
* Residue conservation analysis
PDB id:
2afr
Name: Isomerase
Title: The crystal structure of putative precorrin isomerase cbic i cobalamin biosynthesis
Structure: Cobalamin biosynthesis precorrin isomerase. Chain: a. Engineered: yes
Source: Leptospira interrogans. Organism_taxid: 173. Expressed in: escherichia coli. Expression_system_taxid: 562
Biol. unit: Dimer (from PQS)
Resolution:
2.30Å     R-factor:   0.224     R-free:   0.284
Authors: Y.Xue,Z.Wei,X.Li
Key ref: Y.Xue et al. (2006). The crystal structure of putative precorrin isomerase CbiC in cobalamin biosynthesis. J Struct Biol, 153, 307-311. PubMed id: 16427313 DOI: 10.1016/j.jsb.2005.11.011
Date:
26-Jul-05     Release date:   04-Apr-06    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q8EXP7  (Q8EXP7_LEPIN) -  Cobalt-precorrin-8 methylmutase
Seq:
Struc:
220 a.a.
216 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.5.4.99.60  - Cobalt-precorrin-8 methylmutase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Cobalt-precorrin-8 = cobyrinate
Cobalt-precorrin-8
= cobyrinate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     cobalamin biosynthetic process   1 term 
  Biochemical function     isomerase activity     2 terms  

 

 
    Added reference    
 
 
DOI no: 10.1016/j.jsb.2005.11.011 J Struct Biol 153:307-311 (2006)
PubMed id: 16427313  
 
 
The crystal structure of putative precorrin isomerase CbiC in cobalamin biosynthesis.
Y.Xue, Z.Wei, X.Li, W.Gong.
 
  ABSTRACT  
 
The leptospira cbiC encodes the enzyme catalyzing the methyl rearrangement reaction of the cobalamin biosynthesis pathway. The protein has been cloned and overexpressed as a His-tagged recombinant protein in Escherichia coli. The crystal structures have been solved in two crystal forms (P4(2)2(1)2 and P3(1)21) diffracting to 3.0 and 2.3A resolution, respectively. The structures are similar to the precorrin-8x methyl mutase (CobH), an enzyme of the aerobic pathway to vitamin B12.
 

 

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