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PDBsum entry 1zxk
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Cell adhesion
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PDB id
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1zxk
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* Residue conservation analysis
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PDB id:
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Cell adhesion
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Title:
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Crystal structure of cadherin8 ec1 domain
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Structure:
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Cadherin-8. Chain: a, b. Fragment: ec1 domain. Engineered: yes. Mutation: yes
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Source:
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Mus musculus. House mouse. Organism_taxid: 10090. Gene: cdh8. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Biol. unit:
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Dimer (from
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Resolution:
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2.00Å
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R-factor:
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0.254
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R-free:
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0.283
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Authors:
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S.D.Patel,C.Ciatto,C.P.Chen,F.Bahna,N.Arkus,I.Schieren,M.Rajebhosale, T.M.Jessell,B.Honig,S.R.Price,L.Shapiro
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Key ref:
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S.D.Patel
et al.
(2006).
Type II cadherin ectodomain structures: implications for classical cadherin specificity.
Cell,
124,
1255-1268.
PubMed id:
DOI:
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Date:
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08-Jun-05
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Release date:
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25-Apr-06
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PROCHECK
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Headers
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References
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P97291
(CADH8_MOUSE) -
Cadherin-8 from Mus musculus
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Seq:
Struc:
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799 a.a.
96 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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DOI no:
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Cell
124:1255-1268
(2006)
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PubMed id:
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Type II cadherin ectodomain structures: implications for classical cadherin specificity.
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S.D.Patel,
C.Ciatto,
C.P.Chen,
F.Bahna,
M.Rajebhosale,
N.Arkus,
I.Schieren,
T.M.Jessell,
B.Honig,
S.R.Price,
L.Shapiro.
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ABSTRACT
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Type I and II classical cadherins help to determine the adhesive specificities
of animal cells. Crystal-structure determination of ectodomain regions from
three type II cadherins reveals adhesive dimers formed by exchange of N-terminal
beta strands between partner extracellular cadherin-1 (EC1) domains. These
interfaces have two conserved tryptophan side chains that anchor each swapped
strand, compared with one in type I cadherins, and include large hydrophobic
regions unique to type II interfaces. The EC1 domains of type I and type II
cadherins appear to encode cell adhesive specificity in vitro. Moreover,
perturbation of motor neuron segregation with chimeric cadherins depends on EC1
domain identity, suggesting that this region, which includes the structurally
defined adhesive interface, encodes type II cadherin functional specificity in
vivo.
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Selected figure(s)
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Figure 1.
Figure 1. Structure of Multidomain Type II Cadherin
Fragments
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Figure 4.
Figure 4. Comparison of Binding Surfaces for Type II
Cadherins and Type I Cadherins
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The above figures are
reprinted
by permission from Cell Press:
Cell
(2006,
124,
1255-1268)
copyright 2006.
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Figures were
selected
by an automated process.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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J.Brasch,
O.J.Harrison,
G.Ahlsen,
S.M.Carnally,
R.M.Henderson,
B.Honig,
and
L.Shapiro
(2011).
Structure and binding mechanism of vascular endothelial cadherin: a divergent classical cadherin.
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J Mol Biol,
408,
57-73.
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PDB code:
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J.Vendome,
S.Posy,
X.Jin,
F.Bahna,
G.Ahlsen,
L.Shapiro,
and
B.Honig
(2011).
Molecular design principles underlying β-strand swapping in the adhesive dimerization of cadherins.
|
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Nat Struct Mol Biol,
18,
693-700.
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PDB code:
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Y.Wu,
J.Vendome,
L.Shapiro,
A.Ben-Shaul,
and
B.Honig
(2011).
Transforming binding affinities from three dimensions to two with application to cadherin clustering.
|
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Nature,
475,
510-513.
|
|
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|
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C.Ciatto,
F.Bahna,
N.Zampieri,
H.C.VanSteenhouse,
P.S.Katsamba,
G.Ahlsen,
O.J.Harrison,
J.Brasch,
X.Jin,
S.Posy,
J.Vendome,
B.Ranscht,
T.M.Jessell,
B.Honig,
and
L.Shapiro
(2010).
T-cadherin structures reveal a novel adhesive binding mechanism.
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| |
Nat Struct Mol Biol,
17,
339-347.
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PDB codes:
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D.Schreiner,
and
J.A.Weiner
(2010).
Combinatorial homophilic interaction between gamma-protocadherin multimers greatly expands the molecular diversity of cell adhesion.
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| |
Proc Natl Acad Sci U S A,
107,
14893-14898.
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H.M.Elledge,
P.Kazmierczak,
P.Clark,
J.S.Joseph,
A.Kolatkar,
P.Kuhn,
and
U.Müller
(2010).
Structure of the N terminus of cadherin 23 reveals a new adhesion mechanism for a subset of cadherin superfamily members.
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Proc Natl Acad Sci U S A,
107,
10708-10712.
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PDB code:
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I.Le Trong,
P.Aprikian,
B.A.Kidd,
M.Forero-Shelton,
V.Tchesnokova,
P.Rajagopal,
V.Rodriguez,
G.Interlandi,
R.Klevit,
V.Vogel,
R.E.Stenkamp,
E.V.Sokurenko,
and
W.E.Thomas
(2010).
Structural basis for mechanical force regulation of the adhesin FimH via finger trap-like beta sheet twisting.
|
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Cell,
141,
645-655.
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PDB code:
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M.Fraiberg,
I.Borovok,
R.M.Weiner,
and
R.Lamed
(2010).
Discovery and characterization of cadherin domains in Saccharophagus degradans 2-40.
|
| |
J Bacteriol,
192,
1066-1074.
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|
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|
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M.Sotomayor,
W.A.Weihofen,
R.Gaudet,
and
D.P.Corey
(2010).
Structural determinants of cadherin-23 function in hearing and deafness.
|
| |
Neuron,
66,
85.
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|
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PDB codes:
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O.J.Harrison,
F.Bahna,
P.S.Katsamba,
X.Jin,
J.Brasch,
J.Vendome,
G.Ahlsen,
K.J.Carroll,
S.R.Price,
B.Honig,
and
L.Shapiro
(2010).
Two-step adhesive binding by classical cadherins.
|
| |
Nat Struct Mol Biol,
17,
348-357.
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PDB codes:
|
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Q.Zhou,
M.Kapoor,
M.Guo,
R.Belani,
X.Xu,
W.B.Kiosses,
M.Hanan,
C.Park,
E.Armour,
M.H.Do,
L.A.Nangle,
P.Schimmel,
and
X.L.Yang
(2010).
Orthogonal use of a human tRNA synthetase active site to achieve multifunctionality.
|
| |
Nat Struct Mol Biol,
17,
57-61.
|
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|
|
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|
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S.H.Barnes,
S.R.Price,
C.Wentzel,
and
S.C.Guthrie
(2010).
Cadherin-7 and cadherin-6B differentially regulate the growth, branching and guidance of cranial motor axons.
|
| |
Development,
137,
805-814.
|
|
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S.K.Chang,
Z.Gu,
and
M.B.Brenner
(2010).
Fibroblast-like synoviocytes in inflammatory arthritis pathology: the emerging role of cadherin-11.
|
| |
Immunol Rev,
233,
256-266.
|
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|
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|
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S.Kjaer,
S.Hanrahan,
N.Totty,
and
N.Q.McDonald
(2010).
Mammal-restricted elements predispose human RET to folding impairment by HSCR mutations.
|
| |
Nat Struct Mol Biol,
17,
726-731.
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PDB code:
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T.Padilla-Benavides,
M.L.Roldán,
I.Larre,
D.Flores-Benitez,
N.Villegas-Sepúlveda,
R.G.Contreras,
M.Cereijido,
and
L.Shoshani
(2010).
The polarized distribution of Na+,K+-ATPase: role of the interaction between {beta} subunits.
|
| |
Mol Biol Cell,
21,
2217-2225.
|
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|
|
|
|
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Y.Komarova,
and
A.B.Malik
(2010).
Regulation of endothelial permeability via paracellular and transcellular transport pathways.
|
| |
Annu Rev Physiol,
72,
463-493.
|
|
|
|
|
|
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Y.Wu,
X.Jin,
O.Harrison,
L.Shapiro,
B.H.Honig,
and
A.Ben-Shaul
(2010).
Cooperativity between trans and cis interactions in cadherin-mediated junction formation.
|
| |
Proc Natl Acad Sci U S A,
107,
17592-17597.
|
|
|
|
|
|
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C.McCusker,
H.Cousin,
R.Neuner,
and
D.Alfandari
(2009).
Extracellular cleavage of cadherin-11 by ADAM metalloproteases is essential for Xenopus cranial neural crest cell migration.
|
| |
Mol Biol Cell,
20,
78-89.
|
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|
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J.Kashef,
A.Köhler,
S.Kuriyama,
D.Alfandari,
R.Mayor,
and
D.Wedlich
(2009).
Cadherin-11 regulates protrusive activity in Xenopus cranial neural crest cells upstream of Trio and the small GTPases.
|
| |
Genes Dev,
23,
1393-1398.
|
|
|
|
|
|
|
L.Raptis,
R.Arulanandam,
A.Vultur,
M.Geletu,
S.Chevalier,
and
H.Feracci
(2009).
Beyond structure, to survival: activation of Stat3 by cadherin engagement.
|
| |
Biochem Cell Biol,
87,
835-843.
|
|
|
|
|
|
|
L.Shapiro,
and
W.I.Weis
(2009).
Structure and biochemistry of cadherins and catenins.
|
| |
Cold Spring Harbor Perspect Biol,
1,
a003053.
|
|
|
|
|
|
|
M.Jelani,
M.Salman Chishti,
and
W.Ahmad
(2009).
A novel splice-site mutation in the CDH3 gene in hypotrichosis with juvenile macular dystrophy.
|
| |
Clin Exp Dermatol,
34,
68-73.
|
|
|
|
|
|
|
P.Hulpiau,
and
F.van Roy
(2009).
Molecular evolution of the cadherin superfamily.
|
| |
Int J Biochem Cell Biol,
41,
349-369.
|
|
|
|
|
|
|
P.Katsamba,
K.Carroll,
G.Ahlsen,
F.Bahna,
J.Vendome,
S.Posy,
M.Rajebhosale,
S.Price,
T.M.Jessell,
A.Ben-Shaul,
L.Shapiro,
and
B.H.Honig
(2009).
Linking molecular affinity and cellular specificity in cadherin-mediated adhesion.
|
| |
Proc Natl Acad Sci U S A,
106,
11594-11599.
|
|
|
|
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|
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R.M.Harmon,
B.V.Desai,
and
K.J.Green
(2009).
Regulatory roles of the cadherin superfamily.
|
| |
F1000 Biol Rep,
1,
0.
|
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|
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T.A.Cutler,
B.M.Mills,
D.J.Lubin,
L.T.Chong,
and
S.N.Loh
(2009).
Effect of interdomain linker length on an antagonistic folding-unfolding equilibrium between two protein domains.
|
| |
J Mol Biol,
386,
854-868.
|
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W.Meng,
and
M.Takeichi
(2009).
Adherens junction: molecular architecture and regulation.
|
| |
Cold Spring Harb Perspect Biol,
1,
a002899.
|
|
|
|
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|
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Y.Zhang,
Y.Zhou,
J.Zhu,
S.Dong,
C.Li,
and
Q.Xiang
(2009).
Effect of a novel recombinant protein of fibronectinIII7-10/cadherin 11 EC1-2 on osteoblastic adhesion and differentiation.
|
| |
Biosci Biotechnol Biochem,
73,
1999-2006.
|
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|
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A.Hartsock,
and
W.J.Nelson
(2008).
Adherens and tight junctions: structure, function and connections to the actin cytoskeleton.
|
| |
Biochim Biophys Acta,
1778,
660-669.
|
|
|
|
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|
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C.B.Lira,
K.Chu,
Y.C.Lee,
M.C.Hu,
and
S.H.Lin
(2008).
Expression of the extracellular domain of OB-cadherin as an Fc fusion protein using bicistronic retroviral expression vector.
|
| |
Protein Expr Purif,
61,
220-226.
|
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C.L.Tinkle,
H.A.Pasolli,
N.Stokes,
and
E.Fuchs
(2008).
New insights into cadherin function in epidermal sheet formation and maintenance of tissue integrity.
|
| |
Proc Natl Acad Sci U S A,
105,
15405-15410.
|
|
|
|
|
|
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C.M.Niessen,
and
C.J.Gottardi
(2008).
Molecular components of the adherens junction.
|
| |
Biochim Biophys Acta,
1778,
562-571.
|
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|
|
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|
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H.O.Ishikawa,
H.Takeuchi,
R.S.Haltiwanger,
and
K.D.Irvine
(2008).
Four-jointed is a Golgi kinase that phosphorylates a subset of cadherin domains.
|
| |
Science,
321,
401-404.
|
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I.H.Bekirov,
V.Nagy,
A.Svoronos,
G.W.Huntley,
and
D.L.Benson
(2008).
Cadherin-8 and N-cadherin differentially regulate pre- and postsynaptic development of the hippocampal mossy fiber pathway.
|
| |
Hippocampus,
18,
349-363.
|
|
|
|
|
|
|
L.M.Dibbens,
P.S.Tarpey,
K.Hynes,
M.A.Bayly,
I.E.Scheffer,
R.Smith,
J.Bomar,
E.Sutton,
L.Vandeleur,
C.Shoubridge,
S.Edkins,
S.J.Turner,
C.Stevens,
S.O'Meara,
C.Tofts,
S.Barthorpe,
G.Buck,
J.Cole,
K.Halliday,
D.Jones,
R.Lee,
M.Madison,
T.Mironenko,
J.Varian,
S.West,
S.Widaa,
P.Wray,
J.Teague,
E.Dicks,
A.Butler,
A.Menzies,
A.Jenkinson,
R.Shepherd,
J.F.Gusella,
Z.Afawi,
A.Mazarib,
M.Y.Neufeld,
S.Kivity,
D.Lev,
T.Lerman-Sagie,
A.D.Korczyn,
C.P.Derry,
G.R.Sutherland,
K.Friend,
M.Shaw,
M.Corbett,
H.G.Kim,
D.H.Geschwind,
P.Thomas,
E.Haan,
S.Ryan,
S.McKee,
S.F.Berkovic,
P.A.Futreal,
M.R.Stratton,
J.C.Mulley,
and
J.Gécz
(2008).
X-linked protocadherin 19 mutations cause female-limited epilepsy and cognitive impairment.
|
| |
Nat Genet,
40,
776-781.
|
|
|
|
|
|
|
M.P.Stemmler
(2008).
Cadherins in development and cancer.
|
| |
Mol Biosyst,
4,
835-850.
|
|
|
|
|
|
|
M.Sotomayor,
and
K.Schulten
(2008).
The allosteric role of the Ca2+ switch in adhesion and elasticity of C-cadherin.
|
| |
Biophys J,
94,
4621-4633.
|
|
|
|
|
|
|
S.C.Suzuki,
and
M.Takeichi
(2008).
Cadherins in neuronal morphogenesis and function.
|
| |
Dev Growth Differ,
50,
S119-S130.
|
|
|
|
|
|
|
S.Posy,
L.Shapiro,
and
B.Honig
(2008).
Sequence and structural determinants of strand swapping in cadherin domains: do all cadherins bind through the same adhesive interface?
|
| |
J Mol Biol,
378,
954-968.
|
|
|
|
|
|
|
V.Z.Miloushev,
F.Bahna,
C.Ciatto,
G.Ahlsen,
B.Honig,
L.Shapiro,
and
A.G.Palmer
(2008).
Dynamic properties of a type II cadherin adhesive domain: implications for the mechanism of strand-swapping of classical cadherins.
|
| |
Structure,
16,
1195-1205.
|
|
|
|
|
|
|
A.Al-Amoudi,
D.C.Díez,
M.J.Betts,
and
A.S.Frangakis
(2007).
The molecular architecture of cadherins in native epidermal desmosomes.
|
| |
Nature,
450,
832-837.
|
|
|
|
|
|
|
C.T.Capaldo,
and
I.G.Macara
(2007).
Depletion of E-cadherin disrupts establishment but not maintenance of cell junctions in Madin-Darby canine kidney epithelial cells.
|
| |
Mol Biol Cell,
18,
189-200.
|
|
|
|
|
|
|
D.L.Stokes
(2007).
Desmosomes from a structural perspective.
|
| |
Curr Opin Cell Biol,
19,
565-571.
|
|
|
|
|
|
|
D.P.Corey
(2007).
Stringing the fiddle: the inner ear's two-part invention.
|
| |
Nat Neurosci,
10,
1232-1233.
|
|
|
|
|
|
|
E.Parisini,
J.M.Higgins,
J.H.Liu,
M.B.Brenner,
and
J.H.Wang
(2007).
The crystal structure of human E-cadherin domains 1 and 2, and comparison with other cadherins in the context of adhesion mechanism.
|
| |
J Mol Biol,
373,
401-411.
|
|
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PDB code:
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|
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|
|
G.Agiostratidou,
J.Hulit,
G.R.Phillips,
and
R.B.Hazan
(2007).
Differential cadherin expression: potential markers for epithelial to mesenchymal transformation during tumor progression.
|
| |
J Mammary Gland Biol Neoplasia,
12,
127-133.
|
|
|
|
|
|
|
H.Morishita,
and
T.Yagi
(2007).
Protocadherin family: diversity, structure, and function.
|
| |
Curr Opin Cell Biol,
19,
584-592.
|
|
|
|
|
|
|
L.Shapiro,
J.Love,
and
D.R.Colman
(2007).
Adhesion molecules in the nervous system: structural insights into function and diversity.
|
| |
Annu Rev Neurosci,
30,
451-474.
|
|
|
|
|
|
|
M.R.Akins,
D.L.Benson,
and
C.A.Greer
(2007).
Cadherin expression in the developing mouse olfactory system.
|
| |
J Comp Neurol,
501,
483-497.
|
|
|
|
|
|
|
M.Takeichi
(2007).
The cadherin superfamily in neuronal connections and interactions.
|
| |
Nat Rev Neurosci,
8,
11-20.
|
|
|
|
|
|
|
P.Kazmierczak,
H.Sakaguchi,
J.Tokita,
E.M.Wilson-Kubalek,
R.A.Milligan,
U.Müller,
and
B.Kachar
(2007).
Cadherin 23 and protocadherin 15 interact to form tip-link filaments in sensory hair cells.
|
| |
Nature,
449,
87-91.
|
|
|
|
|
|
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PDB code:
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F.Cailliez,
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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|
Links
