PDBsum entry 1zuz

Metal binding protein/transferase

PDB id: 1zuz

Contents

Protein chains

145 a.a. *

19 a.a. *

Metals

_CA ×4

Waters ×121

* Residue conservation analysis

PDB id:

1zuz

Name:

Metal binding protein/transferase

Title:

Calmodulin in complex with a mutant peptide from human drp-1 kinase

Structure:

Calmodulin. Chain: a. Engineered: yes. Drp-1 kinase. Chain: b. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: synthetic peptide, mutation s308d, the sequence of the peptide: calmodulin-binding domain is naturally found in homo sapiens.

Biol. unit:

Tetramer (from PQS)

Resolution:

1.91Å R-factor: 0.194 R-free: 0.248

Authors:

P.Kursula,J.Vahokoski,M.Wilmanns

Key ref:

P.Kursula et al. Recognition of human death-Associated protein kinases by calmodulin. To be published, .

Date:

01-Jun-05 Release date: 14-Nov-06

Protein chain

P0DP23  (CALM1_HUMAN) -  Calmodulin-1 from Homo sapiens

Seq: 149 a.a.

Struc: 145 a.a.

Protein chain

Q9UIK4  (DAPK2_HUMAN) -  Death-associated protein kinase 2 from Homo sapiens

Seq: 370 a.a.

Struc: 19 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chain B: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site