PDBsum entry 1zdt

Transcription

PDB id: 1zdt

Contents

Protein chains

233 a.a. *

12 a.a. *

11 a.a. *

Ligands

PEF ×2

Waters ×235

* Residue conservation analysis

PDB id:

1zdt

Name:

Transcription

Title:

The crystal structure of human steroidogenic factor-1

Structure:

Steroidogenic factor 1. Chain: a, b. Synonym: stf-1, sf-1, adrenal 4 binding protein, steroid hormone receptor ad4bp, fushi tarazu factor homolog 1. Engineered: yes. Mutation: yes. Nuclear receptor coactivator 2. Chain: p, q. Fragment: residues 741-752.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: this sequence occurs naturally in humans

Biol. unit:

Dimer (from PQS)

Resolution:

2.10Å R-factor: 0.218 R-free: 0.265

Authors:

W.Wang,C.Zhang,A.Marimuthu,H.I.Krupka,M.Tabrizizad,R.Shelloe,U.Mehra, K.Eng,H.Nguyen,C.Settachatgul,B.Powell,M.V.Milburn,B.L.West

Key ref:

W.Wang et al. (2005). The crystal structures of human steroidogenic factor-1 and liver receptor homologue-1. Proc Natl Acad Sci U S A, 102, 7505-7510. PubMed id: 15897460 DOI: 10.1073/pnas.0409482102

Date:

14-Apr-05 Release date: 24-May-05

Protein chains

Q13285  (STF1_HUMAN) -  Steroidogenic factor 1 from Homo sapiens

Seq: 461 a.a.

Struc: 233 a.a.*

Protein chain

Q15596  (NCOA2_HUMAN) -  Nuclear receptor coactivator 2 from Homo sapiens

Seq: 1464 a.a.

Struc: 12 a.a.

Protein chain

Q15596  (NCOA2_HUMAN) -  Nuclear receptor coactivator 2 from Homo sapiens

Seq: 1464 a.a.

Struc: 11 a.a.

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B, P, Q: E.C.?

DOI no: 10.1073/pnas.0409482102

Proc Natl Acad Sci U S A 102:7505-7510 (2005)

PubMed id: 15897460

The crystal structures of human steroidogenic factor-1 and liver receptor homologue-1.

W.Wang, C.Zhang, A.Marimuthu, H.I.Krupka, M.Tabrizizad, R.Shelloe, U.Mehra, K.Eng, H.Nguyen, C.Settachatgul, B.Powell, M.V.Milburn, B.L.West.

ABSTRACT

Steroidogenic factor-1 (SF-1) and liver receptor homologue-1 (LRH-1) belong to the fushi tarazu factor 1 subfamily of nuclear receptors. SF-1 is an essential factor for sex determination during development and regulates adrenal and gonadal steroidogenesis in the adult, whereas LRH-1 is a critical factor for development of endodermal tissues and regulates cholesterol and bile acid homeostasis. Regulatory ligands are unknown for SF-1 and LRH-1. A reported mouse LRH-1 structure revealed an empty pocket in a region commonly occupied by ligands in the structures of other nuclear receptors, and pocket-filling mutations did not alter the constitutive activity observed. Here we report the crystal structures of the putative ligand-binding domains of human SF-1 at 2.1-A resolution and human LRH-1 at 2.5-A resolution. Both structures bind a coactivator-derived peptide at the canonical activation-function surface, thus adopting the transcriptionally activating conformation. In human LRH-1, coactivator peptide binding also occurs to a second site. We discovered in both structures a phospholipid molecule bound in a pocket of the putative ligand-binding domain. MS analysis of the protein samples used for crystallization indicated that the two proteins associate with a range of phospholipids. Mutations of the pocket-lining residues reduced the transcriptional activities of SF-1 and LRH-1 in mammalian cell transfection assays without affecting their expression levels. These results suggest that human SF-1 and LRH-1 may be ligand-binding receptors, although it remains to be seen if phospholipids or possibly other molecules regulate SF-1 or LRH-1 under physiological conditions.

Selected figure(s)

Figure 1.
Fig. 1. The hSF-1 and hLRH-1 LBD structures complexed with phospholipid and coactivator peptide. (A and B) The hSF-1 LBD (A) and the hLRH-1 LBD (B) (gray ribbon models) with phospholipid ligands (spherical model colored by atom type), and NCOA2 coactivator peptide (blue ribbon model). Note that two NCOA2 peptides bind to each LRH-1 molecule, one at the canonical activation function surface and the other at a site formed by H2, H3, and the -sheet. (C) Residues of the hSF-1 ligand-binding pocket (stick models colored yellow and by atom type), showing salt bridge and hydrogen bonds (dotted lines) to the PE (stick models colored white and by atom type). The blue mesh indicates an unbiased 2F[o] - F[c] map covering the ligand. H2 and H3 are truncated to show the pocket features. (D) Residues of the hLRH-1 ligand-binding pocket depicted as in C showing interactions with the phosphatidylglycerol-phosphoglycerol.

Figure 2.
Fig. 2. Comparison of hSF-1 and hLRH-1 structures with mLRH-1. (A and B) A phosphate group in hSF-1 (A) and hLRH-1 (B) interacts with the Lys and Tyr of the KYG triad. (C) E440 in the APO mLRH-1 mimics the phosphate group interactions. Only the residues of the phosphate-binding triad (sticks colored yellow and by atom type) and the polar portions of the phospholipids (sticks colored by atom type) are shown. (D) Alignment of representative NR5A sequences. The two sequence segments that contain the KYG motif (helix H6-H7 and H11) are shown. A more complete alignment appears in Fig. 8.

Figures were selected by an automated process.