PDBsum entry 1zbh

Hydrolase/RNA

PDB id: 1zbh

Contents

Protein chains

289 a.a. *

225 a.a. *

DNA/RNA

Ligands

AMP ×4

Metals

_MG ×8

Waters ×18

* Residue conservation analysis

PDB id:

1zbh

Name:

Hydrolase/RNA

Title:

3'-end specific recognition of histone mRNA stem-loop by 3'- exonuclease

Structure:

5'-r( Cp Cp Gp Gp Cp Up Cp Up Up Up Up Cp Ap Gp Ap Gp Cp Cp Gp G)-3'. Chain: f, e. Fragment: sl-RNA. Engineered: yes. 3'-5' exonuclease eri1. Chain: a, b, c, d. Fragment: 3'hexo. Synonym: eri-1 homolog, histone mRNA 3' end-specific exoribonuclease,

Source:

Synthetic: yes. Homo sapiens. Human. Organism_taxid: 9606. Gene: eri1, 3'exo. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Biol. unit:

Hexamer (from PQS)

Resolution:

3.00Å R-factor: 0.214 R-free: 0.260

Authors:

Y.Cheng,D.J.Patel

Key ref:

Y.Cheng and d.j.patel Structural basis for 3'-End specific recognition of histone mRNA stem-Loop by 3'-Exonuclease, A human nuclease that also targets sirna. To be published, .

Date:

08-Apr-05 Release date: 26-Sep-06

Protein chains

Q8IV48  (ERI1_HUMAN) -  3'-5' exoribonuclease 1 from Homo sapiens

Seq: 349 a.a.

Struc: 289 a.a.*

Protein chains

Q8IV48  (ERI1_HUMAN) -  3'-5' exoribonuclease 1 from Homo sapiens

Seq: 349 a.a.

Struc: 225 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

DNA/RNA chains

G-G-C-U-C-U-U-U-U-C-A-G-A-G-C-C 16 bases

G-G-C-U-C-U-U-U-U-C-A-G-A-G-C-C 16 bases

Enzyme reactions

• Enzyme class: Chains A, B, C, D: E.C.3.1.-.-