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PDBsum entry 1z7z
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Virus/receptor
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PDB id
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1z7z
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Contents |
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214 a.a.
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263 a.a.
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192 a.a.
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12 a.a.
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434 a.a.
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References listed in PDB file
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Key reference
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Title
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The crystal structure of coxsackievirus a21 and its interaction with icam-1.
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Authors
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C.Xiao,
C.M.Bator-Kelly,
E.Rieder,
P.R.Chipman,
A.Craig,
R.J.Kuhn,
E.Wimmer,
M.G.Rossmann.
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Ref.
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Structure, 2005,
13,
1019-1033.
[DOI no: ]
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PubMed id
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Abstract
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CVA21 and polioviruses both belong to the Enterovirus genus in the family of
Picornaviridae, whereas rhinoviruses form a distinct picornavirus genus.
Nevertheless, CVA21 and the major group of human rhinoviruses recognize
intercellular adhesion molecule-1 (ICAM-1) as their cellular receptor, whereas
polioviruses use poliovirus receptor. The crystal structure of CVA21 has been
determined to 3.2 A resolution. Its structure has greater similarity to
poliovirus structures than to other known picornavirus structures. Cryo-electron
microscopy (cryo-EM) was used to determine an 8.0 A resolution structure of
CVA21 complexed with an ICAM-1 variant, ICAM-1(Kilifi). The cryo-EM map was
fitted with the crystal structures of ICAM-1 and CVA21. Significant differences
in the structure of CVA21 with respect to the poliovirus structures account for
the inability of ICAM-1 to bind polioviruses. The interface between CVA21 and
ICAM-1 has shape and electrostatic complementarity with many residues being
conserved among those CVAs that bind ICAM-1.
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Figure 6.
Figure 6. Road Maps of the ICAM-1^Kilifi Contact Area with
CVA21
(A) Stereo diagram of enlarged contact area in Figure
5A.
(B) The viral (left) and ICAM-1^Kilifi (right) residues
that are in the virus/receptor interface are shown on a
two-dimensional projection of the same contact area (orange) as
in (A). The three contour lines represent virus-receptor
separation distances of 2.0 (red), 3.0 (orange), and 4.0
(yellow) Å.
(C) Stereo diagram of the contact area (white)
with surface color based on electrostatic potentials. Positively
and negatively charged surfaces are colored blue and red,
respectively.
(D) Two-dimensional projection of (C) with a
roadmap of corresponding residues of CVA21 (left) and
ICAM-1^Kilifi (right). The four ionic network sites are
encircled with green dashed lines and are labeled with
green-colored letters of a, b, c, and d, corresponding to the
notation used in Figure 7.
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The above figure is
reprinted
by permission from Cell Press:
Structure
(2005,
13,
1019-1033)
copyright 2005.
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