PDBsum entry 1z7k

Hydrolase/hydrolase inhibitor

PDB id: 1z7k

Contents

Protein chains

223 a.a.

62 a.a.

Ligands

THR-ASN-GLU-GLU

NAG-NAG

Waters ×126

References listed in PDB file

Key reference

• Title: Crystal structure of trypsin-Turkey egg white inhibitor complex.
• Authors: B.S.Ibrahim, V.Pattabhi.
• Ref.: Biochem Biophys Res Commun, 2004, 313, 8. [DOI no: 10.1016/j.bbrc.2003.11.082]
• PubMed id: 14672690

Abstract

Crystal structure of the complex between porcine beta-trypsin and the second domain of the Kazal-type ovomucoid turkey egg white trypsin inhibitor (OMTKY2) has been determined at 1.9A resolution. A peptide fragment from the first domain has been crystallized with the complex. Restrained-refinement of the structure led to an R-factor of 0.19 for the 32206 reflections. OMTKY2 exhibits the canonical Kazal-type fold with a central alpha-helix and a short two-stranded anti-parallel beta-sheet. The carbonyl carbon of the reactive site prefers trigonal geometry. The reactive site loop geometry of the inhibitor is complementary to the surface and charge of the binding site in beta-trypsin.