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PDBsum entry 1z64

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protein links
Antimicrobial protein PDB id
1z64
Jmol
Contents
Protein chain
26 a.a.
PDB id:
1z64
Name: Antimicrobial protein
Title: Nmr solution structure of pleurocidin in dpc micelles
Structure: Pleruocidin. Chain: a. Fragment: residues 23-47. Engineered: yes
Source: Pseudopleuronectes americanus. Winter flounder. Organism_taxid: 8265. Expressed in: escherichia coli. Expression_system_taxid: 562.
NMR struc: 11 models
Authors: R.T.Syvitski,I.Burton,N.R.Mattatall,S.E.Douglas,D.L.Jakeman
Key ref:
R.T.Syvitski et al. (2005). Structural characterization of the antimicrobial peptide pleurocidin from winter flounder. Biochemistry, 44, 7282-7293. PubMed id: 15882067 DOI: 10.1021/bi0504005
Date:
21-Mar-05     Release date:   12-Apr-05    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P81941  (PLE1_PSEAM) -  Pleurocidin
Seq:
Struc:
68 a.a.
26 a.a.*
Key:    PfamA domain  Secondary structure
* PDB and UniProt seqs differ at 1 residue position (black cross)

 

 
DOI no: 10.1021/bi0504005 Biochemistry 44:7282-7293 (2005)
PubMed id: 15882067  
 
 
Structural characterization of the antimicrobial peptide pleurocidin from winter flounder.
R.T.Syvitski, I.Burton, N.R.Mattatall, S.E.Douglas, D.L.Jakeman.
 
  ABSTRACT  
 
Pleurocidin is an antimicrobial peptide that was isolated from the mucus membranes of winter flounder (Pseudopleuronectes americanus) and contributes to the initial stages of defense against bacterial infection. From NMR structural studies with the uniformly (15)N-labeled peptide, a structure of pleurocidin was determined to be in a random coil conformation in aqueous solution whereas it assumes an alpha-helical structure in TFE and in dodecylphosphocholine (DPC) micelles. From (15)N relaxation studies, the helix is a rigid structure in the membrane-mimicking environment. Strong NOESY cross-peaks from the pleurocidin to the aliphatic chain on DPC confirm that pleurocidin is contained within the DPC micelle and not associated with the surface of the micelle. From diffusion studies it was determined that each micelle contains at least two pleurocidin molecules.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
19946788 D.M.Santos, R.M.Verly, D.Piló-Veloso, M.de Maria, M.A.de Carvalho, P.S.Cisalpino, B.M.Soares, C.G.Diniz, L.M.Farias, D.F.Moreira, F.Frézard, M.P.Bemquerer, A.M.Pimenta, and M.E.de Lima (2010).
LyeTx I, a potent antimicrobial peptide from the venom of the spider Lycosa erythrognatha.
  Amino Acids, 39, 135-144.  
19330556 S.Subramanian, N.W.Ross, and S.L.MacKinnon (2009).
Myxinidin, a novel antimicrobial peptide from the epidermal mucus of hagfish, Myxine glutinosa L.
  Mar Biotechnol (NY), 11, 748-757.  
  19517207 X.Tian, R.T.Syvitski, T.Liu, N.Livingstone, D.L.Jakeman, and Y.H.Li (2009).
A method for structure-activity analysis of quorum-sensing signaling peptides from naturally transformable streptococci.
  Biol Proced Online, 11, 207-226.  
17766347 A.J.Mason, A.Marquette, and B.Bechinger (2007).
Zwitterionic phospholipids and sterols modulate antimicrobial peptide-induced membrane destabilization.
  Biophys J, 93, 4289-4299.  
17935693 C.M.Gabrys, and D.P.Weliky (2007).
Chemical shift assignment and structural plasticity of a HIV fusion peptide derivative in dodecylphosphocholine micelles.
  Biochim Biophys Acta, 1768, 3225-3234.  
17570764 C.Y.Pan, J.Y.Chen, Y.S.Cheng, C.Y.Chen, I.H.Ni, J.F.Sheen, Y.L.Pan, and C.M.Kuo (2007).
Gene expression and localization of the epinecidin-1 antimicrobial peptide in the grouper (Epinephelus coioides), and its role in protecting fish against pathogenic infection.
  DNA Cell Biol, 26, 403-413.  
16936029 R.T.Syvitski, X.L.Tian, K.Sampara, A.Salman, S.F.Lee, D.L.Jakeman, and Y.H.Li (2007).
Structure-activity analysis of quorum-sensing signaling peptides from Streptococcus mutans.
  J Bacteriol, 189, 1441-1450.
PDB codes: 2i2h 2i2j
16636829 I.Brocal, A.Falco, V.Mas, A.Rocha, L.Perez, J.M.Coll, and A.Estepa (2006).
Stable expression of bioactive recombinant pleurocidin in a fish cell line.
  Appl Microbiol Biotechnol, 72, 1217-1228.  
16736494 N.Y.Yount, A.S.Bayer, Y.Q.Xiong, and M.R.Yeaman (2006).
Advances in antimicrobial peptide immunobiology.
  Biopolymers, 84, 435-458.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.