PDBsum entry 1z1y

Cell adhesion

PDB id: 1z1y

Contents

Protein chains

177 a.a. *

Metals

_YB ×9

Waters ×88

* Residue conservation analysis

PDB id:

1z1y

Name:

Cell adhesion

Title:

Crystal structure of methylated pvs25, an ookinete protein from plasmodium vivax

Structure:

Ookinete surface protein pvs25. Chain: a, b. Engineered: yes

Source:

Plasmodium vivax. Malaria parasite p. Vivax. Organism_taxid: 5855. Expressed in: saccharomyces cerevisiae. Expression_system_taxid: 4932.

Resolution:

2.00Å R-factor: 0.245 R-free: 0.276

Authors:

A.K.Saxena,K.Singh,H.P.Su,M.M.Klein,A.W.Stowers,A.J.Saul,C.A.Long, D.N.Garboczi

Key ref:

A.K.Saxena et al. (2006). The essential mosquito-stage P25 and P28 proteins from Plasmodium form tile-like triangular prisms. Nat Struct Mol Biol, 13, 90-91. PubMed id: 16327807 DOI: 10.1038/nsmb1024

Date:

07-Mar-05 Release date: 06-Dec-05

Protein chains

O96555  (O96555_PLAVI) -  Ookinete surface protein from Plasmodium vivax

Seq: 219 a.a.

Struc: 177 a.a.*

* PDB and UniProt seqs differ at 22 residue positions (black crosses)

DOI no: 10.1038/nsmb1024

Nat Struct Mol Biol 13:90-91 (2006)

PubMed id: 16327807

The essential mosquito-stage P25 and P28 proteins from Plasmodium form tile-like triangular prisms.

A.K.Saxena, K.Singh, H.P.Su, M.M.Klein, A.W.Stowers, A.J.Saul, C.A.Long, D.N.Garboczi.

ABSTRACT

P25 and P28 proteins are essential for Plasmodium parasites to infect mosquitoes and are leading candidates for a transmission-blocking malaria vaccine. The Plasmodium vivax P25 is a triangular prism that could tile the parasite surface. The residues forming the triangle are conserved in P25 and P28 from all Plasmodium species. A cocrystal structure shows that a transmission-blocking antibody uses only its heavy chain to bind Pvs25 at a vertex of the triangle.

Selected figure(s)

Figure 1.
Figure 1. Views of Pvs25. (a) The triangular prism formed by domain 1 (light blue), domain 2 (green), domain 3 (red) and domain 4 (gold). The central -strands in each domain are labeled 1 and 2. The 11 disulfide bonds (violet) are shown. (b) View of the edge of the prism. (c) Pvs25 forms sheets in the crystals. One molecule (red) makes contacts with four 2[1] symmetry mates (light blue) and two molecules related by lattice translations (dark blue). The six light blue molecules all have the same triangular face 'up', whereas the other three molecules (red and dark blue) all have the opposite face 'up'. As P25 and P28 molecules are thin prisms, the glycosylphosphatidylinositol anchor could reach the cell membrane whether a molecule was facing 'up' or 'down' on the membrane. N term, N terminus; C term, C terminus.

Figure 2.
Figure 2. Binding of transmission-blocking antibodies to Pvs25. (a) 2A8 Fab bound via its heavy chain (dark blue) to the B loop of Pvs25 domain 2. The light chain (light blue) does not contact Pvs25. The B loop of Pvs25 domain 3 (red) extends up from the plane of the triangle. (b) Monoclonal antibodies 1H10 and 1A5 do not bind in the presence of prebound 2A8. With Pvs25 immobilized on a Biacore chip, 2A8 was injected at (i) until binding was saturated at (ii), and then the second antibody and 2A8 were injected at (iii) until (iv). Buffer without protein was injected from (ii) to (iii) and from (iv) onward.

The above figures are reprinted by permission from Macmillan Publishers Ltd: Nat Struct Mol Biol (2006, 13, 90-91) copyright 2006.