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PDBsum entry 1yui
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DNA binding protein/DNA
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PDB id
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1yui
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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The solution structure of a specific gaga factor-Dna complex reveals a modular binding mode.
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Authors
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J.G.Omichinski,
P.V.Pedone,
G.Felsenfeld,
A.M.Gronenborn,
G.M.Clore.
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Ref.
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Nat Struct Biol, 1997,
4,
122-132.
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PubMed id
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Abstract
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The structure of a complex between the DNA binding domain of the GAGA factor
(GAGA-DBD) and an oligonucleotide containing its GAGAG consensus binding site
has been determined by nuclear magnetic resonance spectroscopy. The GAGA-DBD
comprises a single classical Cys2-His2 zinc finger core, and an N-terminal
extension containing two highly basic regions, BR1 and BR2. The zinc finger core
binds in the major groove and recognizes the first three GAG bases of the
consensus in a manner similar to that seen in other classical zinc finger-DNA
complexes. Unlike the latter, which require tandem zinc finger repeats with a
minimum of two units for high affinity binding, the GAGA-DBD makes use of only a
single finger complemented by BR1 and BR2. BR2 forms a helix that interacts in
the major groove recognizing the last G of the consensus, while BR1 wraps around
the DNA in the minor groove and recognizes the A in the fourth position of the
consensus. The implications of the structure of the GAGA-DBD-DNA complex for
chromatin remodelling are discussed.
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