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UniProt functional annotation for P37821 | ||
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| UniProt code: P37821. |
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| Organism: | |
Malus domestica (Apple) (Pyrus malus). |
| Taxonomy: | |
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Maleae; Malus. |
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| Function: | |
Catalyzes the formation of 1-aminocyclopropane-1-carboxylate, a direct precursor of ethylene in higher plants (PubMed:7809054, PubMed:11591146). Catalyzes also the conversion of L-vinylglycine (L- VG) to alpha-ketobutyrate and ammonia (PubMed:10704193). Can use S- methylmethionine as substrate (PubMed:14678788). {ECO:0000269|PubMed:10704193, ECO:0000269|PubMed:11591146, ECO:0000269|PubMed:14678788, ECO:0000269|PubMed:7809054}. |
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| Catalytic activity: | |
Reaction=S-adenosyl-L-methionine = 1-aminocyclopropane-1-carboxylate + H(+) + S-methyl-5'-thioadenosine; Xref=Rhea:RHEA:21744, ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:58360, ChEBI:CHEBI:59789; EC=4.4.1.14; Evidence={ECO:0000269|PubMed:11591146, ECO:0000269|PubMed:7809054}; |
| Catalytic activity: | |
Reaction=(2S)-2-amino-3-butenoate + H2O = 2-oxobutanoate + NH4(+); Xref=Rhea:RHEA:51916, ChEBI:CHEBI:15377, ChEBI:CHEBI:16763, ChEBI:CHEBI:28938, ChEBI:CHEBI:134467; Evidence={ECO:0000269|PubMed:10704193}; |
| Cofactor: | |
Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326; Evidence={ECO:0000269|PubMed:7809054, ECO:0000269|PubMed:9398277}; |
| Activity regulation: | |
Inhibited by L-aminoethoxyvinylglycine (AVG) (PubMed:12228256). Inhibited by L-vinylglycine (L-VG) (PubMed:10704193). Inhibited by S-methylmethionine through a L-VG ketimine intermediate (PubMed:14678788). {ECO:0000269|PubMed:10704193, ECO:0000269|PubMed:12228256, ECO:0000269|PubMed:14678788}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=12 uM for S-adenosyl-L-methionine {ECO:0000269|PubMed:7809054}; KM=1.4 mM for L-vinylglycine {ECO:0000269|PubMed:10704193}; KM=4 mM for S-methylmethionine {ECO:0000269|PubMed:14678788}; Note=kcat is 9 sec(-1) per monomer with S-adenosyl-L-methionine as substrate (PubMed:7809054). kcat is 1.8 sec(-1) with L-vinylglycine as substrate (PubMed:10704193). kcat is 2.7 min(-1) with S- methylmethionine as substrate (PubMed:14678788). {ECO:0000269|PubMed:10704193, ECO:0000269|PubMed:14678788, ECO:0000269|PubMed:7809054}; |
| Pathway: | |
Alkene biosynthesis; ethylene biosynthesis via S-adenosyl-L- methionine; ethylene from S-adenosyl-L-methionine: step 1/2. {ECO:0000305}. |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:12228256, ECO:0000269|PubMed:7809054}. |
| Developmental stage: | |
Expressed during ripening. {ECO:0000269|PubMed:24186277}. |
| Similarity: | |
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.