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PDBsum entry 1ycj
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Membrane protein
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PDB id
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1ycj
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References listed in PDB file
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Key reference
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Title
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Crystal structure of the kainate receptor glur5 ligand-Binding core in complex with (s)-Glutamate.
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Authors
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P.Naur,
B.Vestergaard,
L.K.Skov,
J.Egebjerg,
M.Gajhede,
J.S.Kastrup.
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Ref.
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FEBS Lett, 2005,
579,
1154-1160.
[DOI no: ]
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PubMed id
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Abstract
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The X-ray structure of the ligand-binding core of the kainate receptor GluR5
(GluR5-S1S2) in complex with (S)-glutamate was determined to 1.95 A resolution.
The overall GluR5-S1S2 structure comprises two domains and is similar to the
related AMPA receptor GluR2-S1S2J. (S)-glutamate binds as in GluR2-S1S2J.
Distinct features are observed for Ser741, which stabilizes a highly coordinated
network of water molecules and forms an interdomain bridge. The GluR5 complex
exhibits a high degree of domain closure (26 degrees) relative to apo
GluR2-S1S2J. In addition, GluR5-S1S2 forms a novel dimer interface with a
different arrangement of the two protomers compared to GluR2-S1S2J.
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Figure 1.
Fig. 1. Ligand-binding cores of the three classes of
iGluRs. Cartoon representations of the overall structures of the
AMPA receptor GluR2-S1S2J (MolB, pdb code 1FTJ; left figure),
the kainate receptor GluR5-S1S2 (MolB; middle figure) and the
NMDA receptor NR1-S1S2 (MolA, pdb code 1PB7; right figure).
Domain D1 (primarily composed of S1 residues) is colored cyan
and D2 (primarily composed of S2 residues) is colored brown.
GluR2-S1S2J and GluR5-S1S2 were crystallized in the presence of
(S)-glutamate, whereas NR1 was crystallized in complex with
(S)-glycine. The ligands are shown in ball-and-stick
representation.
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Figure 2.
Fig. 2. Comparison of the structures of GluR5-S1S2 and
GluR2-S1S2J. (A) Structural alignment of GluR5-S1S2 and
GluR2-S1S2J. Boxes correspond to structurally conserved regions.
The Gly-Thr linker is shaded grey. (B) Superimposition of the D1
Cα-atoms of the structures of GluR5-S1S2 and GluR2-S1S2J. A
Cα-trace of the two structures is shown in stereo, with
GluR5-S1S2 coloured in green and GluR2-S1S2J in magenta. Every
10th residue of the GluR5-S1S2 structure is labeled.
(S)-glutamate is shown in ball-and-stick.
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The above figures are
reprinted
by permission from the Federation of European Biochemical Societies:
FEBS Lett
(2005,
579,
1154-1160)
copyright 2005.
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