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UniProt functional annotation for Q980Q4 | ||
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| UniProt code: Q980Q4. |
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| Organism: | |
Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus). |
| Taxonomy: | |
Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae; Saccharolobus. |
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| Function: | |
Catalyzes the conversion of uracil and 5-phospho-alpha-D- ribose 1-diphosphate (PRPP) to UMP and diphosphate. {ECO:0000269|PubMed:15752360}. |
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| Catalytic activity: | |
Reaction=diphosphate + UMP = 5-phospho-alpha-D-ribose 1-diphosphate + uracil; Xref=Rhea:RHEA:13017, ChEBI:CHEBI:17568, ChEBI:CHEBI:33019, ChEBI:CHEBI:57865, ChEBI:CHEBI:58017; EC=2.4.2.9; Evidence={ECO:0000269|PubMed:15752360}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250}; Note=Binds 1 Mg(2+) ion per subunit. The magnesium is bound as Mg-PRPP. {ECO:0000250}; |
| Activity regulation: | |
Allosterically activated by GTP. Inhibited by CTP and UMP in combination. {ECO:0000269|PubMed:15752360}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=55 uM for 5-phospho-alpha-D-ribose 1-diphosphate (in the presence of 1 mM GTP) {ECO:0000269|PubMed:15752360}; KM=19 uM for 5-phospho-alpha-D-ribose 1-diphosphate (without GTP) {ECO:0000269|PubMed:15752360}; KM=27 uM for uracil (in the presence of 1 mM GTP) {ECO:0000269|PubMed:15752360}; KM=1.4 uM for uracil (without GTP) {ECO:0000269|PubMed:15752360}; Vmax=15.8 umol/min/mg enzyme (in the presence of 1 mM GTP) {ECO:0000269|PubMed:15752360}; Vmax=0.92 umol/min/mg enzyme (without GTP) {ECO:0000269|PubMed:15752360}; pH dependence: Optimum pH is 5.0-5.6 at 60 degrees Celsius. {ECO:0000269|PubMed:15752360}; |
| Pathway: | |
Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1. |
| Subunit: | |
Homotetramer. {ECO:0000269|PubMed:15752360}. |
| Similarity: | |
Belongs to the UPRTase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.