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PDBsum entry 1xii
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Isomerase(intramolecular oxidoreductase)
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PDB id
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1xii
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References listed in PDB file
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Key reference
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Title
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Modes of binding substrates and their analogues to the enzyme d-Xylose isomerase.
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Authors
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H.L.Carrell,
H.Hoier,
J.P.Glusker.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 1994,
50,
113-123.
[DOI no: ]
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PubMed id
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Note In the PDB file this reference is
annotated as "TO BE PUBLISHED".
The citation details given above were identified by an automated
search of PubMed on title and author
names, giving a
perfect match.
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Abstract
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Studies of binding of substrates and inhibitors of the enzyme D-xylose isomerase
show, from X-ray diffraction data at 1.6-1.9 A resolution, that there are a
variety of binding modes. These vary in the manner in which the substrate or its
analogue extend, on binding, across the carboxy end of the (betaalpha)(8)-barrel
structure. These binding sites are His54 and the metal ion (magnesium or
manganese) that is held in place by Glul81, Asp245, Glu217 and Asp287. Possible
catalytic groups have been identified in proposed mechanisms and their role in
the binding of ligands is illustrated.
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Figure 4.
Fig. 4. Binding of D-glucose. (a) Ball-and-stick view and (b)
identification of some binding groups.
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Figure 10.
Fig. 10. Binding of D-alkylation product (Carrell
e/al.,
1989). (a)
Ball-and-stick view and (b) identification of some binding
groups.
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The above figures are
reprinted
by permission from the IUCr:
Acta Crystallogr D Biol Crystallogr
(1994,
50,
113-123)
copyright 1994.
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Secondary reference #1
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Title
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X-Ray analysis of d-Xylose isomerase at 1.9 a: native enzyme in complex with substrate and with a mechanism-Designed inactivator.
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Authors
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H.L.Carrell,
J.P.Glusker,
V.Burger,
F.Manfre,
D.Tritsch,
J.F.Biellmann.
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Ref.
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Proc Natl Acad Sci U S A, 1989,
86,
4440-4444.
[DOI no: ]
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PubMed id
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Secondary reference #2
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Title
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Comparison of backbone structures of glucose isomerase from streptomyces and arthrobacter.
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Authors
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K.Henrick,
D.M.Blow,
H.L.Carrell,
J.P.Glusker.
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Ref.
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Protein Eng, 1987,
1,
467-469.
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PubMed id
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Secondary reference #3
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Title
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X-Ray crystal structure of d-Xylose isomerase at 4-A resolution.
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Authors
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H.L.Carrell,
B.H.Rubin,
T.J.Hurley,
J.P.Glusker.
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Ref.
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J Biol Chem, 1984,
259,
3230-3236.
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PubMed id
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