PDBsum entry 1xig

Go to PDB code: 
Top Page protein ligands metals links
Isomerase(intramolecular oxidoreductase) PDB id
Protein chain
385 a.a.
_MN ×2
Waters ×296

References listed in PDB file
Key reference
Title Modes of binding substrates and their analogues to the enzyme d-Xylose isomerase.
Authors H.L.Carrell, H.Hoier, J.P.Glusker.
Ref. Acta Crystallogr D Biol Crystallogr, 1994, 50, 113-123. [DOI no: 10.1107/S0907444993009345]
PubMed id 15299449
Note In the PDB file this reference is annotated as "TO BE PUBLISHED". The citation details given above were identified by an automated search of PubMed on title and author names, giving a perfect match.
Studies of binding of substrates and inhibitors of the enzyme D-xylose isomerase show, from X-ray diffraction data at 1.6-1.9 A resolution, that there are a variety of binding modes. These vary in the manner in which the substrate or its analogue extend, on binding, across the carboxy end of the (betaalpha)(8)-barrel structure. These binding sites are His54 and the metal ion (magnesium or manganese) that is held in place by Glul81, Asp245, Glu217 and Asp287. Possible catalytic groups have been identified in proposed mechanisms and their role in the binding of ligands is illustrated.
Figure 4.
Fig. 4. Binding of D-glucose. (a) Ball-and-stick view and (b) identification of some binding groups.
Figure 10.
Fig. 10. Binding of D-alkylation product (Carrell e/al., 1989). (a) Ball-and-stick view and (b) identification of some binding groups.
The above figures are reprinted by permission from the IUCr: Acta Crystallogr D Biol Crystallogr (1994, 50, 113-123) copyright 1994.
Secondary reference #1
Title X-Ray analysis of d-Xylose isomerase at 1.9 a: native enzyme in complex with substrate and with a mechanism-Designed inactivator.
Authors H.L.Carrell, J.P.Glusker, V.Burger, F.Manfre, D.Tritsch, J.F.Biellmann.
Ref. Proc Natl Acad Sci U S A, 1989, 86, 4440-4444. [DOI no: 10.1073/pnas.86.12.4440]
PubMed id 2734296
Full text Abstract
Secondary reference #2
Title Comparison of backbone structures of glucose isomerase from streptomyces and arthrobacter.
Authors K.Henrick, D.M.Blow, H.L.Carrell, J.P.Glusker.
Ref. Protein Eng, 1987, 1, 467-469.
PubMed id 3508294
Secondary reference #3
Title X-Ray crystal structure of d-Xylose isomerase at 4-A resolution.
Authors H.L.Carrell, B.H.Rubin, T.J.Hurley, J.P.Glusker.
Ref. J Biol Chem, 1984, 259, 3230-3236.
PubMed id 6699015
Go to PROCHECK summary