PDBsum entry 1x6l

Hydrolase

PDB id: 1x6l

Contents

Protein chain

539 a.a. *

Waters ×383

* Residue conservation analysis

PDB id:

1x6l

Name:

Hydrolase

Title:

Crystal structure of s. Marcescens chitinase a mutant w167a

Structure:

Chitinase a. Chain: a. Engineered: yes. Mutation: yes

Source:

Serratia marcescens. Organism_taxid: 615. Gene: chia. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.90Å R-factor: 0.217 R-free: 0.233

Authors:

N.N.Aronson Jr.,B.A.Halloran,M.F.Alexyev,X.E.Zhou,Y.Wang,E.J.Meehan, L.Chen

Key ref:

N.N.Aronson jr. et al. Mutation of trp167 at the -3 subsite of the chitin-Binding cleft of s. Marcescens chitinase a caused enhanced transglycosylation. To be published, .

Date:

11-Aug-04 Release date: 26-Jul-05

Protein chain

P07254  (CHIA_SERMA) -  Chitinase A from Serratia marcescens

Seq: 563 a.a.

Struc: 539 a.a.*

* PDB and UniProt seqs differ at 8 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.2.1.14 - chitinase.
• Reaction: Hydrolysis of the 1,4-beta-linkages of N-acetyl-D-glucosamine polymers of chitin.