UniProt functional annotation for P55345



	UniProt functional annotation for P55345

UniProt code: P55345.

Organism: Homo sapiens (Human).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.

Function: Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)- mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation. May inhibit NF-kappa-B transcription and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1- dependent manner). May be involved in growth regulation. {ECO:0000269|PubMed:12039952, ECO:0000269|PubMed:16648481, ECO:0000269|PubMed:17587566, ECO:0000269|PubMed:19405910}.

Catalytic activity: Reaction=L-arginyl-[protein] + 2 S-adenosyl-L-methionine = 2 H(+) + N(omega),N(omega)-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L- homocysteine; Xref=Rhea:RHEA:48096, Rhea:RHEA-COMP:10532, Rhea:RHEA- COMP:11991, ChEBI:CHEBI:15378, ChEBI:CHEBI:29965, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:61897; EC=2.1.1.319; Evidence={ECO:0000269|PubMed:19405910};

Biophysicochemical properties: Kinetic parameters: KM=2.6 uM for AdoMet {ECO:0000269|PubMed:19405910}; KM=3.3 uM for H4 {ECO:0000269|PubMed:19405910}; Vmax=1.4 pmol/min/mg enzyme toward S-adenosyl-L-methionine (AdoMet) {ECO:0000269|PubMed:19405910}; Vmax=1.5 pmol/min/mg enzyme toward histone H4 {ECO:0000269|PubMed:19405910};

Subunit: Self-associates. Interacts with RB1 and E2F1 (By similarity). Interacts with NCOA6 coactivator. Interacts (via SH3 domain) with PRMT8. Interacts with AR. Interacts with NFKBIA. Interacts with ESR1, ESR2, PGR, PPARG, RARA, RXRA and THRB. Interacts with HNRNPUL1. {ECO:0000250, ECO:0000269|PubMed:11513728, ECO:0000269|PubMed:12039952, ECO:0000269|PubMed:16648481, ECO:0000269|PubMed:17587566, ECO:0000269|PubMed:17925405, ECO:0000269|PubMed:22093364}.

Subcellular location: [Isoform 1]: Cytoplasm. Nucleus. Note=Translocates from the cytoplasm to the nucleus, after hormone exposure. Excluded from nucleolus.

Subcellular location: [Isoform PRMT2Alpha]: Nucleus. Note=Excluded from nucleolus.

Subcellular location: [Isoform PRMT2Beta]: Cytoplasm. Nucleus. Nucleus, nucleolus.

Subcellular location: [Isoform PRMT2Gamma]: Nucleus. Note=Excluded from nucleolus.

Subcellular location: [Isoform PRMT2L2]: Cytoplasm {ECO:0000269|PubMed:21820040}. Nucleus {ECO:0000269|PubMed:21820040}. Note=Predominantly cytoplasmic.

Tissue specificity: Widely expressed. Highly expressed in androgen target organs such as heart, prostate, skeletal muscle, ovary and spinal cord. {ECO:0000269|PubMed:17587566}.

Miscellaneous: [Isoform PRMT2Alpha]: Higher expression in breast cancer tissues. {ECO:0000305}.

Miscellaneous: [Isoform PRMT2Beta]: Higher expression in breast cancer tissues. Doesn't interact with ESR1. {ECO:0000305}.

Miscellaneous: [Isoform PRMT2Gamma]: Higher expression in breast cancer tissues. {ECO:0000305}.

Similarity: Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. {ECO:0000255|PROSITE-ProRule:PRU01015}.

Annotations taken from UniProtKB at the EBI.


Organism:		Homo sapiens (Human).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.



Function:		Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)- mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation. May inhibit NF-kappa-B transcription and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1- dependent manner). May be involved in growth regulation. {ECO:0000269\|PubMed:12039952, ECO:0000269\|PubMed:16648481, ECO:0000269\|PubMed:17587566, ECO:0000269\|PubMed:19405910}.


Catalytic activity:		Reaction=L-arginyl-[protein] + 2 S-adenosyl-L-methionine = 2 H(+) + N(omega),N(omega)-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L- homocysteine; Xref=Rhea:RHEA:48096, Rhea:RHEA-COMP:10532, Rhea:RHEA- COMP:11991, ChEBI:CHEBI:15378, ChEBI:CHEBI:29965, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:61897; EC=2.1.1.319; Evidence={ECO:0000269\|PubMed:19405910};
Biophysicochemical properties:		Kinetic parameters: KM=2.6 uM for AdoMet {ECO:0000269\|PubMed:19405910}; KM=3.3 uM for H4 {ECO:0000269\|PubMed:19405910}; Vmax=1.4 pmol/min/mg enzyme toward S-adenosyl-L-methionine (AdoMet) {ECO:0000269\|PubMed:19405910}; Vmax=1.5 pmol/min/mg enzyme toward histone H4 {ECO:0000269\|PubMed:19405910};
Subunit:		Self-associates. Interacts with RB1 and E2F1 (By similarity). Interacts with NCOA6 coactivator. Interacts (via SH3 domain) with PRMT8. Interacts with AR. Interacts with NFKBIA. Interacts with ESR1, ESR2, PGR, PPARG, RARA, RXRA and THRB. Interacts with HNRNPUL1. {ECO:0000250, ECO:0000269\|PubMed:11513728, ECO:0000269\|PubMed:12039952, ECO:0000269\|PubMed:16648481, ECO:0000269\|PubMed:17587566, ECO:0000269\|PubMed:17925405, ECO:0000269\|PubMed:22093364}.
Subcellular location:		[Isoform 1]: Cytoplasm. Nucleus. Note=Translocates from the cytoplasm to the nucleus, after hormone exposure. Excluded from nucleolus.
Subcellular location:		[Isoform PRMT2Alpha]: Nucleus. Note=Excluded from nucleolus.
Subcellular location:		[Isoform PRMT2Beta]: Cytoplasm. Nucleus. Nucleus, nucleolus.
Subcellular location:		[Isoform PRMT2Gamma]: Nucleus. Note=Excluded from nucleolus.
Subcellular location:		[Isoform PRMT2L2]: Cytoplasm {ECO:0000269\|PubMed:21820040}. Nucleus {ECO:0000269\|PubMed:21820040}. Note=Predominantly cytoplasmic.
Tissue specificity:		Widely expressed. Highly expressed in androgen target organs such as heart, prostate, skeletal muscle, ovary and spinal cord. {ECO:0000269\|PubMed:17587566}.
Miscellaneous:		[Isoform PRMT2Alpha]: Higher expression in breast cancer tissues. {ECO:0000305}.
Miscellaneous:		[Isoform PRMT2Beta]: Higher expression in breast cancer tissues. Doesn't interact with ESR1. {ECO:0000305}.
Miscellaneous:		[Isoform PRMT2Gamma]: Higher expression in breast cancer tissues. {ECO:0000305}.
Similarity:		Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. {ECO:0000255\|PROSITE-ProRule:PRU01015}.