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UniProt functional annotation for Q99405 | ||
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| UniProt code: Q99405. |
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| Organism: | |
Bacillus clausii (strain KSM-K16). |
| Taxonomy: | |
Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalobacillus. |
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| Function: | |
Alkaline serine protease that cleaves various substrates, including N-succinyl-Ala-Ala-Pro-Phe-pNA, N-succinyl-Ala-Ala-Pro- MetpNA, oxidized insulin B chain, casein, hemoglobin and scleroproteins, such as keratin, alpha-keratin and elastin. {ECO:0000269|PubMed:7632397}. |
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| Cofactor: | |
Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000269|PubMed:15299321}; Note=Binds 2 calcium ions per subunit. {ECO:0000269|PubMed:15299321}; |
| Activity regulation: | |
Activity is inhibited by phenylmethylsulfonyl fluoride and chymostatin. {ECO:0000269|PubMed:7632397}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.8 mM for N-succinyl-Ala-Ala-Pro-Phe-pNA {ECO:0000269|PubMed:7632397}; KM=1.01 mM for N-succinyl-Ala-Ala-Pro-Met-pNA {ECO:0000269|PubMed:7632397}; pH dependence: Optimum pH is 12.3. {ECO:0000269|PubMed:7632397}; Temperature dependence: Optimum temperature is 55 degrees Celsius. CaC1(2) markedly shifts the optimum temperature to 70 degrees Celsius. {ECO:0000269|PubMed:7632397}; |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:15299321}. |
| Subcellular location: | |
Secreted {ECO:0000305|PubMed:7632397}. |
| Similarity: | |
Belongs to the peptidase S8 family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.