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PDBsum entry 1wru

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protein links
Structural protein PDB id
1wru
Jmol
Contents
Protein chain
325 a.a. *
Waters ×180
* Residue conservation analysis
PDB id:
1wru
Name: Structural protein
Title: Structure of central hub elucidated by x-ray analysis of gen 44; baseplate component of bacteriophage mu
Structure: 43 kda tail protein. Chain: a. Synonym: gpp, gene product 44. Engineered: yes
Source: Enterobacteria phage mu. Organism_taxid: 10677. Gene: 44. Expressed in: escherichia coli. Expression_system_taxid: 562.
Biol. unit: Trimer (from PDB file)
Resolution:
2.10Å     R-factor:   0.222     R-free:   0.249
Authors: Y.Kondou,D.Kitazawa,S.Takeda,E.Yamashita,M.Mizuguchi,K.Kawan T.Tsukihara
Key ref:
Y.Kondou et al. (2005). Crystallization and preliminary X-ray analysis of gene product 44 from bacteriophage Mu. Acta Crystallograph Sect F Struct Biol Cryst Commun, 61, 104-105. PubMed id: 16508104 DOI: 10.1107/S1744309104029574
Date:
27-Oct-04     Release date:   20-Sep-05    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P08558  (VPP_BPMU) -  Baseplate hub protein gp44
Seq:
Struc:
379 a.a.
325 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     virion   2 terms 
  Biological process     release of virus from host   2 terms 

 

 
DOI no: 10.1107/S1744309104029574 Acta Crystallograph Sect F Struct Biol Cryst Commun 61:104-105 (2005)
PubMed id: 16508104  
 
 
Crystallization and preliminary X-ray analysis of gene product 44 from bacteriophage Mu.
Y.Kondou, D.Kitazawa, S.Takeda, E.Yamashita, M.Mizuguchi, K.Kawano, T.Tsukihara.
 
  ABSTRACT  
 
Bacteriophage Mu baseplate protein gene product 44 (gp44) is an essential protein required for the assembly of viable phages. To investigate the roles of gp44 in baseplate assembly and infection, gp44 was crystallized at pH 6.0 in the presence of 20% 2-methyl-2,4-pentanediol. The crystals belong to space group R3, with unit-cell parameters a = b = 127.47, c = 63.97 A. The crystals diffract X-rays to at least 2.1 A resolution and are stable in the X-ray beam and are therefore appropriate for structure determination. Native data have been collected to 2.1 A resolution using a DIP6040 image-plate system at beamline BL44XU at the SPring-8 facility in Japan.
 
  Selected figure(s)  
 
Figure 1.
Figure 1 Photomicrograph of a gp44 crystal. The largest crystal dimension was approximately 0.8 mm.
 
  The above figure is reprinted from an Open Access publication published by the IUCr: Acta Crystallograph Sect F Struct Biol Cryst Commun (2005, 61, 104-105) copyright 2005.