PDBsum entry 1wr3

Ligase

PDB id: 1wr3

Contents

Protein chain

36 a.a. *

* Residue conservation analysis

PDB id:

1wr3

Name:

Ligase

Title:

Solution structure of the first ww domain of nedd4-2

Structure:

Ubiquitin-protein ligase nedd4-2. Chain: a. Fragment: first ww domain. Engineered: yes

Source:

Mus musculus. House mouse. Organism_taxid: 10090. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.

NMR struc:

20 models

Authors:

K.Kowalski,A.L.Merkel,G.W.Booker

Key ref:

K.Kowalski et al. Solution structures of ww domains of nedd4-2. To be published, .

Date:

11-Oct-04 Release date: 18-Oct-05

Protein chain

Q8CFI0  (NED4L_MOUSE) -  E3 ubiquitin-protein ligase NEDD4-like from Mus musculus

Seq: 1004 a.a.

Struc: 36 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: E.C.2.3.2.26 - HECT-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine
• Enzyme class 2: E.C.2.3.2.36 - RING-type E3 ubiquitin transferase (cysteine targeting).
• Reaction: [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-S-ubiquitinyl-L-cysteine

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.