UniProt functional annotation for P50876



	UniProt functional annotation for P50876

UniProt code: P50876.

Organism: Homo sapiens (Human).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.

Function: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates the ubiquitination and degradation of the DNA damage kinase PRKDC. {ECO:0000250, ECO:0000269|PubMed:24979766}.

Catalytic activity: Reaction=[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.; EC=2.3.2.31; Evidence={ECO:0000250|UniProtKB:O60260};

Pathway: Protein modification; protein ubiquitination.

Subunit: Interacts with UBE2L3. {ECO:0000269|PubMed:10431818}.

Subcellular location: Cell membrane {ECO:0000269|PubMed:24979766}; Single-pass membrane protein {ECO:0000305}. Cytoplasmic vesicle membrane {ECO:0000269|PubMed:24979766}.

Domain: Members of the RBR family are atypical E3 ligases. They interact with the E2 conjugating enzyme UBE2L3 and function like HECT- type E3 enzymes: they bind E2s via the first RING domain, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved cysteine residue in the second RING domain. {ECO:0000250|UniProtKB:O60260}.

Ptm: Autoubiquitinated. {ECO:0000269|PubMed:24979766}.

Similarity: Belongs to the RBR family. RNF144 subfamily. {ECO:0000305}.

Sequence caution: Sequence=BAA11478.2; Type=Erroneous initiation; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.


Organism:		Homo sapiens (Human).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.



Function:		E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates the ubiquitination and degradation of the DNA damage kinase PRKDC. {ECO:0000250, ECO:0000269\|PubMed:24979766}.


Catalytic activity:		Reaction=[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.; EC=2.3.2.31; Evidence={ECO:0000250\|UniProtKB:O60260};
Pathway:		Protein modification; protein ubiquitination.
Subunit:		Interacts with UBE2L3. {ECO:0000269\|PubMed:10431818}.
Subcellular location:		Cell membrane {ECO:0000269\|PubMed:24979766}; Single-pass membrane protein {ECO:0000305}. Cytoplasmic vesicle membrane {ECO:0000269\|PubMed:24979766}.
Domain:		Members of the RBR family are atypical E3 ligases. They interact with the E2 conjugating enzyme UBE2L3 and function like HECT- type E3 enzymes: they bind E2s via the first RING domain, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved cysteine residue in the second RING domain. {ECO:0000250\|UniProtKB:O60260}.
Ptm:		Autoubiquitinated. {ECO:0000269\|PubMed:24979766}.
Similarity:		Belongs to the RBR family. RNF144 subfamily. {ECO:0000305}.
Sequence caution:		Sequence=BAA11478.2; Type=Erroneous initiation; Evidence={ECO:0000305};