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PDBsum entry 1w7z
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Protease inhibitor
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PDB id
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1w7z
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References listed in PDB file
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Key reference
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Title
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Structure of ecballium elaterium trypsin inhibitor ii (eeti-Ii): a rigid molecular scaffold.
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Authors
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R.Krätzner,
J.E.Debreczeni,
T.Pape,
T.R.Schneider,
A.Wentzel,
H.Kolmar,
G.M.Sheldrick,
I.Uson.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 2005,
61,
1255-1262.
[DOI no: ]
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PubMed id
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Abstract
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The Ecballium elaterium trypsin inhibitor II (EETI-II) belongs to the family of
squash inhibitors and is one of the strongest inhibitors known for trypsin. The
eight independent molecules of EETI-II in the crystal structure reported here
provide a good opportunity to test the hypothesis that this small cystine-knot
protein (knottin) is sufficiently rigid to be used as a molecular scaffold for
protein-engineering purposes. To extend this test, the structures of two
complexes of EETI-II with trypsin have also been determined, one carrying a
four-amino-acid mutation of EETI-II. The remarkable similarity of these
structures confirms the rigidity of the molecular framework and hence its
suitability as a molecular scaffold.
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Figure 3.
Figure 3
Dimer formed by the molecules A and B. Molecule pairs CD and EF display a similar
arrangement. The sodium ion is shown as a solid ball.
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Figure 6.
Figure 6
Least-squares superpositions of all main-chain atoms of EETI-II. (a) All eight independent
molecules in the uncomplexed structure; (b) uncomplexed (black) and complexed (red)
EETI-II and the NMR model (green); (c) uncomplexed EETI-II (black), the trypsin-EETI-II-
[beta] TNNK complex (red) and CMTI-I (blue).
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The above figures are
reprinted
by permission from the IUCr:
Acta Crystallogr D Biol Crystallogr
(2005,
61,
1255-1262)
copyright 2005.
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