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UniProt functional annotation for P50232 | ||
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| UniProt code: P50232. |
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| Organism: | |
Rattus norvegicus (Rat). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. |
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| Function: | |
Synaptotagmin family member which does not bind Ca(2+) (PubMed:7993622). Involved in neuronal dense core vesicles (DCVs) mobility through its interaction with KIF1A. Upon increased neuronal activity, phosphorylation by MAPK8/JNK1 destabilizes the interaction with KIF1A and captures DCVs to synapses (PubMed:29166604). Plays a role in dendrite formation by melanocytes (By similarity). {ECO:0000250|UniProtKB:Q9H2B2, ECO:0000269|PubMed:29166604, ECO:0000269|PubMed:7993622}. |
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| Cofactor: | |
Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000255|PROSITE-ProRule:PRU00041}; |
| Subunit: | |
Interacts with KIF1A; the interaction increases in presence of calcium and decreases when SYT4 is phosphorylated at Ser-135. {ECO:0000269|PubMed:29166604, ECO:0000269|PubMed:30021165}. |
| Subcellular location: | |
Cytoplasmic vesicle, secretory vesicle, neuronal dense core vesicle membrane {ECO:0000269|PubMed:29166604, ECO:0000305|PubMed:30021165}; Single-pass membrane protein {ECO:0000305}. |
| Tissue specificity: | |
Widely expressed (PubMed:7892240). Expressed in the brain (PubMed:7892240). Expressed in pituitary gland, cerebellum, cortex, hypothalamus and hippocampus (PubMed:7892240). {ECO:0000269|PubMed:7892240, ECO:0000269|PubMed:7993622}. |
| Induction: | |
Up-regulated by potassium depolarization, calcium ionophore, ATP and forskolin in culture cells (PubMed:7892240). Up-regulated by kainate in the hippocampus and piriform cortex (PubMed:7892240). {ECO:0000269|PubMed:7892240}. |
| Domain: | |
Unlike in other synaptotagmin family members, the first C2 domain/C2A does not bind Ca(2+) neither mediates Ca(2+)-dependent phospholipid binding. An aspartate-to-serine substitution in this domain inactivates Ca(2+)/phospho-lipid binding. {ECO:0000269|PubMed:7993622, ECO:0000269|PubMed:9162066}. |
| Ptm: | |
Phosphorylation at Ser-135 by MAPK8/JNK1 reduces interaction with KIF1A and neuronal dense core vesicles mobility. {ECO:0000269|PubMed:29166604}. |
| Similarity: | |
Belongs to the synaptotagmin family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.