UniProt functional annotation for Q9VUQ5



	UniProt functional annotation for Q9VUQ5

UniProt code: Q9VUQ5.

Organism: Drosophila melanogaster (Fruit fly).

Taxonomy: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora.

Function: Essential for RNA interference (RNAi); double-stranded RNA induces potent and specific gene silencing (PubMed:11498593, PubMed:12368261, PubMed:14508492, PubMed:32504809). RNAi is mediated by the RNA-induced silencing complex (RISC), a sequence-specific, multicomponent nuclease that destroys or silences messenger RNAs homologous to the silencing trigger (PubMed:11498593, PubMed:12368261, PubMed:14508492). {ECO:0000269|PubMed:11498593, ECO:0000269|PubMed:12368261, ECO:0000269|PubMed:14508492, ECO:0000269|PubMed:32504809}.

Cofactor: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};

Subunit: Interacts with Fmr1, Dcr-1 and vig to form the RNA-induced silencing complex (RISC), a ribonucleoprotein (RNP) complex involved in translation regulation, other components of the complex are RpL5, RpL11 and Rm62 (PubMed:11498593, PubMed:14508492, PubMed:12368261). As part of the RISC complex, interacts with Tudor-SN (PubMed:14508492). {ECO:0000269|PubMed:11498593, ECO:0000269|PubMed:12368261, ECO:0000269|PubMed:14508492}.

Subunit: (Microbial infection) Interacts with cricket paralysis virus protein 1A; this interaction may block the RISC activity. {ECO:0000269|PubMed:20400949}.

Subcellular location: Cytoplasm, Cytoplasmic ribonucleoprotein granule {ECO:0000269|PubMed:17178403}.

Domain: PAZ domain provides a major contribution for nucleic acid recognition. PAZ binds oligonucleotides of different lengths and has a strong preference for single-stranded nucleic acids (ssRNA or SSDNA) or RNA duplexes with single-stranded 3' overhangs. Can bind the characteristic two-base 3' overhangs of siRNAs, indicating that it may contribute to the specific and productive incorporation of siRNAs and miRNAs into the RNAi pathway.

Similarity: Belongs to the argonaute family. Ago subfamily. {ECO:0000305}.

Sequence caution: Sequence=AAM11104.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.


Organism:		Drosophila melanogaster (Fruit fly).
Taxonomy:		Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora.



Function:		Essential for RNA interference (RNAi); double-stranded RNA induces potent and specific gene silencing (PubMed:11498593, PubMed:12368261, PubMed:14508492, PubMed:32504809). RNAi is mediated by the RNA-induced silencing complex (RISC), a sequence-specific, multicomponent nuclease that destroys or silences messenger RNAs homologous to the silencing trigger (PubMed:11498593, PubMed:12368261, PubMed:14508492). {ECO:0000269\|PubMed:11498593, ECO:0000269\|PubMed:12368261, ECO:0000269\|PubMed:14508492, ECO:0000269\|PubMed:32504809}.


Cofactor:		Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
Subunit:		Interacts with Fmr1, Dcr-1 and vig to form the RNA-induced silencing complex (RISC), a ribonucleoprotein (RNP) complex involved in translation regulation, other components of the complex are RpL5, RpL11 and Rm62 (PubMed:11498593, PubMed:14508492, PubMed:12368261). As part of the RISC complex, interacts with Tudor-SN (PubMed:14508492). {ECO:0000269\|PubMed:11498593, ECO:0000269\|PubMed:12368261, ECO:0000269\|PubMed:14508492}.
Subunit:		(Microbial infection) Interacts with cricket paralysis virus protein 1A; this interaction may block the RISC activity. {ECO:0000269\|PubMed:20400949}.
Subcellular location:		Cytoplasm, Cytoplasmic ribonucleoprotein granule {ECO:0000269\|PubMed:17178403}.
Domain:		PAZ domain provides a major contribution for nucleic acid recognition. PAZ binds oligonucleotides of different lengths and has a strong preference for single-stranded nucleic acids (ssRNA or SSDNA) or RNA duplexes with single-stranded 3' overhangs. Can bind the characteristic two-base 3' overhangs of siRNAs, indicating that it may contribute to the specific and productive incorporation of siRNAs and miRNAs into the RNAi pathway.
Similarity:		Belongs to the argonaute family. Ago subfamily. {ECO:0000305}.
Sequence caution:		Sequence=AAM11104.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};