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UniProt functional annotation for Q9FNG3 | ||
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| UniProt code: Q9FNG3. |
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| Organism: | |
Arabidopsis thaliana (Mouse-ear cress). |
| Taxonomy: | |
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. |
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| Function: | |
3'-to-5' exoribonuclease (RNase) specifically targeting single-stranded RNAs (PubMed:28463111). Triggers miRNA accumulation in RNA-induced silencing complex (RISC), composed of miRNAs and AGO proteins, by degrading uridylated cleavage fragments (PubMed:28463111). Required during plant growth and development (By similarity). {ECO:0000250|UniProtKB:Q9SF21, ECO:0000269|PubMed:28463111}. |
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| Catalytic activity: | |
Reaction=Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.; EC=3.1.13.1; Evidence={ECO:0000269|PubMed:28463111}; |
| Subunit: | |
Homohexamer with DnaQ-like exonuclease fold in a ring-shaped structure with a central cavity (PubMed:28463111, PubMed:23616405, PubMed:17850744). Component of AGO1 and AGO10-centered RNA-induced silencing complexes (RISC) (PubMed:28463111). Interacts with and acts as a cofactor of AGO1 and AGO10 (PubMed:28463111). {ECO:0000269|PubMed:17850744, ECO:0000269|PubMed:23616405, ECO:0000269|PubMed:28463111}. |
| Subcellular location: | |
Cytoplasm {ECO:0000250|UniProtKB:Q9SF21}. |
| Tissue specificity: | |
Restricted to shoot and root apical meristems, trichomes, and vascular veins. {ECO:0000269|PubMed:28463111}. |
| Disruption phenotype: | |
Little effect on miRNAs levels (PubMed:28463111). Plants lacking both RICE1 and RICE2 have reduced miRNAs levels and lower miRNA retained by AGO1, thus leading to the up-regulation of targeted mRNA transcripts (PubMed:28463111). {ECO:0000269|PubMed:28463111}. |
| Similarity: | |
Belongs to the RICE family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.