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UniProt functional annotation for P0ABU5 | ||
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| UniProt code: P0ABU5. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Displays glyoxalase activity, catalyzing the conversion of glyoxal to glycolate (PubMed:26678554). However, this apparent glyoxalase activity may reflect a protein deglycase activity, which could be the primary function of this protein like other DJ-1 superfamily members such as PARK7, YajL, YhbO and HchA (Probable). Is not able to use methylglyoxal as substrate (PubMed:26678554). {ECO:0000269|PubMed:26678554, ECO:0000305}. |
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| Catalytic activity: | |
Reaction=glyoxal + H2O = glycolate + H(+); Xref=Rhea:RHEA:51672, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29805, ChEBI:CHEBI:34779; Evidence={ECO:0000269|PubMed:26678554}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=1.21 mM for glyoxal (at pH 7.4 and 37 degrees Celsius) {ECO:0000269|PubMed:26678554}; Note=kcat is 0.48 min(-1) with glyoxal as substrate (at pH 7.4 and 37 degrees Celsius). {ECO:0000269|PubMed:26678554}; |
| Subunit: | |
Homodimer. {ECO:0000305|PubMed:16021622, ECO:0000305|Ref.7}. |
| Similarity: | |
Belongs to the peptidase C56 family. {ECO:0000305}. |
| Sequence caution: | |
Sequence=AAA58011.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305}; |
Annotations taken from UniProtKB at the EBI.