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UniProt functional annotation for P40343 | ||
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| UniProt code: P40343. |
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| Organism: | |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. |
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| Function: | |
Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB) and recruits ESCRT-I to the MVB outer membrane (PubMed:3062374, PubMed:1493335, PubMed:9265642, PubMed:11416128, PubMed:12055639, PubMed:11872141, PubMed:12900393, PubMed:14581452, PubMed:15166140, PubMed:15107463, PubMed:15086794, PubMed:17135292, PubMed:18508771). Controls exit from the prevacuolar compartment (PVC) in both the forward direction to the vacuole and the return to the Golgi (PubMed:11208109, PubMed:8649377, PubMed:9015300). Allows VPS10 to return to the (trans-Golgi network) TGN from the PVC (PubMed:8649377). Might also function as an alternate adapter in the COPIb clathrin-like coat (PubMed:17101773). {ECO:0000269|PubMed:11208109, ECO:0000269|PubMed:11416128, ECO:0000269|PubMed:11872141, ECO:0000269|PubMed:12055639, ECO:0000269|PubMed:12900393, ECO:0000269|PubMed:14581452, ECO:0000269|PubMed:1493335, ECO:0000269|PubMed:15086794, ECO:0000269|PubMed:15107463, ECO:0000269|PubMed:15166140, ECO:0000269|PubMed:17101773, ECO:0000269|PubMed:17135292, ECO:0000269|PubMed:18508771, ECO:0000269|PubMed:3062374, ECO:0000269|PubMed:8649377, ECO:0000269|PubMed:9015300, ECO:0000269|PubMed:9265642}. |
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| Subunit: | |
Component of the ESCRT-0 complex composed of HSE1 and VPS27 (PubMed:12055639, PubMed:14581452, PubMed:15086794). Interacts with ENT3 and ENT5, the ESCRT-I subunits VPS23 and VPS28 and with the COPIb subunits SEC27, SEC28 and SEC33 (PubMed:12900393, PubMed:15107463, PubMed:17101773). May form a complex composed of VPS27, HSE1 and DOA1 (PubMed:18508771). Interacts with DOA1 (PubMed:18508771). Interacts with ubiquitin (PubMed:11988742, PubMed:12750381, PubMed:14581452). {ECO:0000269|PubMed:11988742, ECO:0000269|PubMed:12055639, ECO:0000269|PubMed:12750381, ECO:0000269|PubMed:12900393, ECO:0000269|PubMed:14581452, ECO:0000269|PubMed:15086794, ECO:0000269|PubMed:15107463, ECO:0000269|PubMed:17101773, ECO:0000269|PubMed:18508771}. |
| Subcellular location: | |
Endosome membrane {ECO:0000269|PubMed:12006563, ECO:0000269|PubMed:12900393, ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:15169871, ECO:0000269|PubMed:17101773, ECO:0000269|PubMed:17101785}; Peripheral membrane protein {ECO:0000269|PubMed:12006563, ECO:0000269|PubMed:12900393, ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:15169871, ECO:0000269|PubMed:17101773, ECO:0000269|PubMed:17101785}; Cytoplasmic side {ECO:0000269|PubMed:12006563, ECO:0000269|PubMed:12900393, ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:15169871, ECO:0000269|PubMed:17101773, ECO:0000269|PubMed:17101785}. |
| Domain: | |
The FYVE domain is involved in the binding to phosphatidylinositol 3-phosphate (PtdIns(3)P) which is required for the association to endosomal membranes. |
| Domain: | |
Both IUM domains are necessary for efficient binding to ubiquitin. |
| Miscellaneous: | |
Present with 172 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
| Similarity: | |
Belongs to the VPS27 family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.