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UniProt functional annotation for O66728 | ||
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| UniProt code: O66728. |
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| Organism: | |
Aquifex aeolicus (strain VF5). |
| Taxonomy: | |
Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex. |
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| Function: | |
tRNA nucleotidyltransferase involved in the synthesis of the tRNA CCA terminus. Adds the terminal adenosine residue to tRNA (PubMed:11701927, PubMed:25914059). Can incorporate CMP into tRNA ending with C74C75 (tRNACC), with very weak efficiency (PubMed:25914059). {ECO:0000269|PubMed:11701927, ECO:0000269|PubMed:25914059}. |
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| Catalytic activity: | |
Reaction=a tRNA with a 3' CC end + ATP = a tRNA with a 3' CCA end + diphosphate; Xref=Rhea:RHEA:60012, Rhea:RHEA-COMP:10468, Rhea:RHEA- COMP:15488, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:83069, ChEBI:CHEBI:83071; Evidence={ECO:0000269|PubMed:11701927, ECO:0000269|PubMed:25914059}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60013; Evidence={ECO:0000269|PubMed:11701927, ECO:0000269|PubMed:25914059}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000305|PubMed:25914059}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=98 uM for ATP {ECO:0000269|PubMed:25914059}; KM=220 uM for CTP {ECO:0000269|PubMed:25914059}; Note=kcat is 0.10 min(-1) for incorporation of AMP into tRNACC. kcat is 0.00015 min(-1) for incorporation of CMP into tRNACC. {ECO:0000269|PubMed:25914059}; |
| Domain: | |
Adopts a seahorse-like shape and consists of four domains: head, neck, body, and tail. {ECO:0000269|PubMed:25914059, ECO:0000305|PubMed:15295603}. |
| Similarity: | |
Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.