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PDBsum entry 1vee
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Structural genomics, unknown function
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PDB id
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1vee
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References listed in PDB file
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Key reference
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Title
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Solution structure of the rhodanese homology domain at4g01050(175-295) from arabidopsis thaliana.
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Authors
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D.Pantoja-Uceda,
B.López-Méndez,
S.Koshiba,
M.Inoue,
T.Kigawa,
T.Terada,
M.Shirouzu,
A.Tanaka,
M.Seki,
K.Shinozaki,
S.Yokoyama,
P.Güntert.
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Ref.
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Protein Sci, 2005,
14,
224-230.
[DOI no: ]
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PubMed id
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Abstract
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The three-dimensional structure of the rhodanese homology domain
At4g01050(175-195) from Arabidopsis thaliana has been determined by solution
nuclear magnetic resonance methods based on 3043 upper distance limits derived
from NOE intensities measured in three-dimensional NOESY spectra. The structure
shows a backbone root mean square deviation to the mean coordinates of 0.43 A
for the structured residues 7-125. The fold consists of a central parallel
beta-sheet with five strands in the order 1-5-4-2-3 and arranged in the
conventional counterclockwise twist, and helices packing against each side of
the beta-sheet. Comparison with the sequences of other proteins with a rhodanese
homology domain in Arabidopsis thaliana indicated residues that could play an
important role in the scaffold of the rhodanese homology domain. Finally, a
three-dimensional structure comparison of the present noncatalytic rhodanese
homology domain with the noncatalytic rhodanese domains of sulfurtransferases
from other organisms discloses differences in the length and conformation of
loops that could throw light on the role of the noncatalytic rhodanese domain in
sulfurtransferases.
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Figure 1.
Figure 1. Structure-based sequence alignment of the
rhodanese and rhodanese homology domains with known structure
according to the program DALI (Holm and Sander 1996). Domain
names are as in Table 2 Go- . The
catalytic position is shown in italics. Residues in helices or
 -strands are
highlighted in green and in cyan, respectively, and  -helices and
-strands are
labeled.
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The above figure is
reprinted
by permission from the Protein Society:
Protein Sci
(2005,
14,
224-230)
copyright 2005.
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Secondary reference #1
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Title
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Nmr assignment of the hypothetical rhodanese domain at4g01050 from arabidopsis thaliana.
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Authors
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D.Pantoja-Uceda,
B.López-Méndez,
S.Koshiba,
T.Kigawa,
M.Shirouzu,
T.Terada,
M.Inoue,
T.Yabuki,
M.Aoki,
E.Seki,
T.Matsuda,
H.Hirota,
M.Yoshida,
A.Tanaka,
T.Osanai,
M.Seki,
K.Shinozaki,
S.Yokoyama,
P.Güntert.
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Ref.
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J Biomol Nmr, 2004,
29,
207-208.
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PubMed id
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