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PDBsum entry 1vbk
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Structural genomics, unknown function
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PDB id
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1vbk
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References listed in PDB file
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Key reference
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Title
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Purification, Crystallization and preliminary crystallographic analysis of the putative thiamine-Biosynthesis protein ph1313 from pyrococcus horikoshii ot3.
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Authors
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M.Sugahara,
S.Murai,
M.Sugahara,
N.Kunishima.
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Ref.
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Acta Crystallogr Sect F Struct Biol Cryst Commun, 2007,
63,
56-58.
[DOI no: ]
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PubMed id
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Abstract
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The putative thiamine-biosynthesis protein PH1313 from Pyrococcus horikoshii OT3
has been overexpressed and purified. Crystallization was performed by the
oil-microbatch method using 28%(v/v) 2-methyl-2,4-pentanediol as a precipitant
at 291 K. A native X-ray diffraction data set at 1.9 A resolution and a single
anomalous dispersion data set from a selenomethionine-derivative crystal at 2.1
A resolution were collected using synchrotron radiation at 100 K. The native
crystal belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell
parameters a = 71.7, b = 71.2, c = 141.8 A.
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Figure 1.
Crystals of PH1313 from P. horikoshii OT3. The crystals have
approximate dimensions of 0.15 x 0.10 x 0.01 mm. Acta
Crystallogr Sect F Struct Biol Cryst Commun. 2007 January 1;
63(Pt 1): 56–58. Published online 2006 December 22. doi:
10.1107/S1744309106054509. Copyright [copyright] International
Union of Crystallography 2007
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The above figure is
reprinted
from an Open Access publication published by the IUCr:
Acta Crystallogr Sect F Struct Biol Cryst Commun
(2007,
63,
56-58)
copyright 2007.
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