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PDBsum entry 1va2
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Transcription
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PDB id
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1va2
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Contents |
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* Residue conservation analysis
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DOI no:
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Biochemistry
43:16027-16035
(2004)
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PubMed id:
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NMR structure of transcription factor Sp1 DNA binding domain.
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S.Oka,
Y.Shiraishi,
T.Yoshida,
T.Ohkubo,
Y.Sugiura,
Y.Kobayashi.
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ABSTRACT
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To understand the DNA recognition mechanism of zinc finger motifs of
transcription factor Sp1, we have determined the solution structure of
DNA-binding domain of the Sp1 by solution NMR techniques. The DNA-binding domain
of Sp1 consists of three Cys(2)His(2)-type zinc finger motifs. They have typical
betabetaalpha zinc finger folds and relatively random orientations. From
DNA-binding analysis performed by NMR and comparison between structures
determined here and previously reported structures of other zinc fingers, it was
assumed that DNA recognition modes of fingers 2 and 3 would be similar to those
of fingers of Zif268, in which each finger recognizes four base pairs strictly
by using residues at positions -1, 2, 3, and 6 of the recognition helix. On the
contrary, finger 1 can use only two residues for DNA recognition, Lys550 and
His553 at positions -1 and 3 of the helix, and has more relaxed sequence and
site specificity than other Cys(2)His(2) zinc fingers. It is proposed that this
relaxed property of finger 1 allows transcription factor Sp1 to bind various DNA
sequences with high affinity.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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C.C.Chou,
M.Rajasekaran,
and
C.Chen
(2010).
An effective approach for generating a three-Cys2His2 zinc-finger-DNA complex model by docking.
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BMC Bioinformatics,
11,
334.
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J.E.Reid,
K.J.Evans,
N.Dyer,
L.Wernisch,
and
S.Ott
(2010).
Variable structure motifs for transcription factor binding sites.
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BMC Genomics,
11,
30.
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R.K.Kothinti,
A.B.Blodgett,
D.H.Petering,
and
N.M.Tabatabai
(2010).
Cadmium down-regulation of kidney Sp1 binding to mouse SGLT1 and SGLT2 gene promoters: possible reaction of cadmium with the zinc finger domain of Sp1.
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Toxicol Appl Pharmacol,
244,
254-262.
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R.Kothinti,
A.Blodgett,
N.M.Tabatabai,
and
D.H.Petering
(2010).
Zinc finger transcription factor Zn3-Sp1 reactions with Cd2+.
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Chem Res Toxicol,
23,
405-412.
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A.Nomura,
and
A.Okamoto
(2009).
Photoresponsive tandem zinc finger peptide.
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Chem Commun (Camb),
(),
1906-1908.
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X.Zu,
L.Yu,
Q.Sun,
F.Liu,
J.Wang,
Z.Xie,
Y.Wang,
W.Xu,
and
Y.Jiang
(2009).
SP1 enhances Zbtb7A gene expression via direct binding to GC box in HePG2 cells.
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BMC Res Notes,
2,
175.
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A.I.Anzellotti,
and
N.P.Farrell
(2008).
Zinc metalloproteins as medicinal targets.
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Chem Soc Rev,
37,
1629-1651.
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U.Rana,
R.Kothinti,
J.Meeusen,
N.M.Tabatabai,
S.Krezoski,
and
D.H.Petering
(2008).
Zinc binding ligands and cellular zinc trafficking: apo-metallothionein, glutathione, TPEN, proteomic zinc, and Zn-Sp1.
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J Inorg Biochem,
102,
489-499.
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Y.Osawa,
K.Ikebukuro,
H.Motoki,
T.Matsuo,
M.Horiuchi,
and
K.Sode
(2008).
The simple and rapid detection of specific PCR products from bacterial genomes using Zn finger proteins.
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Nucleic Acids Res,
36,
e68.
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J.R.Keys,
M.R.Tallack,
D.J.Hodge,
S.O.Cridland,
R.David,
and
A.C.Perkins
(2007).
Genomic organisation and regulation of murine alpha haemoglobin stabilising protein by erythroid Kruppel-like factor.
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Br J Haematol,
136,
150-157.
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S.J.Bruce,
B.B.Gardiner,
L.J.Burke,
M.M.Gongora,
S.M.Grimmond,
and
A.C.Perkins
(2007).
Dynamic transcription programs during ES cell differentiation towards mesoderm in serum versus serum-freeBMP4 culture.
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BMC Genomics,
8,
365.
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A.M.Pilon,
D.G.Nilson,
D.Zhou,
J.Sangerman,
T.M.Townes,
D.M.Bodine,
and
P.G.Gallagher
(2006).
Alterations in expression and chromatin configuration of the alpha hemoglobin-stabilizing protein gene in erythroid Kruppel-like factor-deficient mice.
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Mol Cell Biol,
26,
4368-4377.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
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