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UniProt functional annotation for Q9ZHI0 | ||
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| UniProt code: Q9ZHI0. |
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| Organism: | |
Thermus filiformis. |
| Taxonomy: | |
Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus. |
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| Function: | |
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double- stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. {ECO:0000255|HAMAP-Rule:MF_01588, ECO:0000269|PubMed:15268945}. |
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| Catalytic activity: | |
Reaction=NAD(+) + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho- (deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + beta- nicotinamide D-nucleotide.; EC=6.5.1.2; Evidence={ECO:0000255|HAMAP- Rule:MF_01588}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:15268945}; |
| Biophysicochemical properties: | |
Temperature dependence: Optimum temperature is 70 degrees Celsius. {ECO:0000269|PubMed:15268945}; |
| Similarity: | |
Belongs to the NAD-dependent DNA ligase family. LigA subfamily. {ECO:0000255|HAMAP-Rule:MF_01588}. |
Annotations taken from UniProtKB at the EBI.