UniProt functional annotation for O35987



	UniProt functional annotation for O35987

UniProt code: O35987.

Organism: Rattus norvegicus (Rat).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus.

Function: Reduces the ATPase activity of VCP (PubMed:9824302, PubMed:9214505). Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis (PubMed:12411482). May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER) (PubMed:10930451). Inhibits the activity of CTSL (in vitro) (By similarity). Together with UBXN2B/p37, regulates the centrosomal levels of kinase AURKA/Aurora A during mitotic progression by promoting AURKA removal from centrosomes in prophase (By similarity). Also, regulates spindle orientation during mitosis (By similarity). {ECO:0000250|UniProtKB:Q9UNZ2, ECO:0000269|PubMed:10930451, ECO:0000269|PubMed:12411482, ECO:0000269|PubMed:9214505, ECO:0000269|PubMed:9824302}.

Subunit: Part of a ternary complex containing STX5A, NSFL1C and VCP. NSFL1C forms a homotrimer that binds to one end of a VCP homohexamer. The complex binds to membranes enriched in phosphatidylethanolamine- containing lipids and promotes Golgi membrane fusion. Interaction with VCIP135 leads to dissociation of the complex via ATP hydrolysis by VCP. Binds ubiquitin and mono-ubiquitinated proteins via its N-terminal UBA- like domain when bound to VCP. {ECO:0000269|PubMed:10811609, ECO:0000269|PubMed:11478859, ECO:0000269|PubMed:12146947, ECO:0000269|PubMed:12473691, ECO:0000269|PubMed:14988733, ECO:0000269|PubMed:15029246, ECO:0000269|PubMed:9214505, ECO:0000269|PubMed:9506515}.

Subcellular location: Nucleus {ECO:0000269|PubMed:12810701}. Golgi apparatus, Golgi stack {ECO:0000269|PubMed:12810701, ECO:0000269|PubMed:9214505}. Chromosome {ECO:0000269|PubMed:1495983}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:23649807}. Note=Predominantly nuclear in interphase cells (PubMed:12810701). Bound to the axial elements of sex chromosomes in pachytene spermatocytes (PubMed:1495983). A small proportion of the protein is cytoplasmic, associated with Golgi stacks (PubMed:12810701). Localizes to centrosome during mitotic prophase and metaphase (PubMed:23649807). {ECO:0000269|PubMed:12810701, ECO:0000269|PubMed:1495983, ECO:0000269|PubMed:23649807}.

Tissue specificity: Highly expressed in heart, brain, spleen, lung, liver, muscle, kidney and testis. {ECO:0000269|PubMed:1495983, ECO:0000269|PubMed:9214505, ECO:0000269|PubMed:9297509}.

Developmental stage: Highly expressed in pachytene spermatocytes during spermatogenesis.

Ptm: Phosphorylated during mitosis. Phosphorylation inhibits interaction with Golgi membranes and is required for the fragmentation of the Golgi stacks during mitosis. {ECO:0000269|PubMed:12810701}.

Similarity: Belongs to the NSFL1C family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Rattus norvegicus (Rat).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus.



Function:		Reduces the ATPase activity of VCP (PubMed:9824302, PubMed:9214505). Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis (PubMed:12411482). May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER) (PubMed:10930451). Inhibits the activity of CTSL (in vitro) (By similarity). Together with UBXN2B/p37, regulates the centrosomal levels of kinase AURKA/Aurora A during mitotic progression by promoting AURKA removal from centrosomes in prophase (By similarity). Also, regulates spindle orientation during mitosis (By similarity). {ECO:0000250\|UniProtKB:Q9UNZ2, ECO:0000269\|PubMed:10930451, ECO:0000269\|PubMed:12411482, ECO:0000269\|PubMed:9214505, ECO:0000269\|PubMed:9824302}.


Subunit:		Part of a ternary complex containing STX5A, NSFL1C and VCP. NSFL1C forms a homotrimer that binds to one end of a VCP homohexamer. The complex binds to membranes enriched in phosphatidylethanolamine- containing lipids and promotes Golgi membrane fusion. Interaction with VCIP135 leads to dissociation of the complex via ATP hydrolysis by VCP. Binds ubiquitin and mono-ubiquitinated proteins via its N-terminal UBA- like domain when bound to VCP. {ECO:0000269\|PubMed:10811609, ECO:0000269\|PubMed:11478859, ECO:0000269\|PubMed:12146947, ECO:0000269\|PubMed:12473691, ECO:0000269\|PubMed:14988733, ECO:0000269\|PubMed:15029246, ECO:0000269\|PubMed:9214505, ECO:0000269\|PubMed:9506515}.
Subcellular location:		Nucleus {ECO:0000269\|PubMed:12810701}. Golgi apparatus, Golgi stack {ECO:0000269\|PubMed:12810701, ECO:0000269\|PubMed:9214505}. Chromosome {ECO:0000269\|PubMed:1495983}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269\|PubMed:23649807}. Note=Predominantly nuclear in interphase cells (PubMed:12810701). Bound to the axial elements of sex chromosomes in pachytene spermatocytes (PubMed:1495983). A small proportion of the protein is cytoplasmic, associated with Golgi stacks (PubMed:12810701). Localizes to centrosome during mitotic prophase and metaphase (PubMed:23649807). {ECO:0000269\|PubMed:12810701, ECO:0000269\|PubMed:1495983, ECO:0000269\|PubMed:23649807}.
Tissue specificity:		Highly expressed in heart, brain, spleen, lung, liver, muscle, kidney and testis. {ECO:0000269\|PubMed:1495983, ECO:0000269\|PubMed:9214505, ECO:0000269\|PubMed:9297509}.
Developmental stage:		Highly expressed in pachytene spermatocytes during spermatogenesis.
Ptm:		Phosphorylated during mitosis. Phosphorylation inhibits interaction with Golgi membranes and is required for the fragmentation of the Golgi stacks during mitosis. {ECO:0000269\|PubMed:12810701}.
Similarity:		Belongs to the NSFL1C family. {ECO:0000305}.