UniProt functional annotation for Q922S8



	UniProt functional annotation for Q922S8

UniProt code: Q922S8.

Organism: Mus musculus (Mouse).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus.

Function: In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells (PubMed:14980225). Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis. Plays a role in chromosome congression and is required for the lateral to end-on conversion of the chromosome-microtubule attachment (By similarity). {ECO:0000250|UniProtKB:Q99661, ECO:0000269|PubMed:14980225}.

Subunit: Interacts with CENPH. Interacts with MTUS2/TIP150; the interaction is direct. Interacts with MAPRE1; the interaction is direct, regulated by phosphorylation and is probably required for targeting to growing microtubule plus ends. Interacts with KIF18B at microtubule tips; this interaction increases the affinity of both partners for microtubule plus ends and is required for robust microtubule depolymerization. Phosphorylation by AURKA or AURKB strongly reduces KIF18B-binding. {ECO:0000250|UniProtKB:Q99661}.

Subcellular location: Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q99661}. Nucleus {ECO:0000250|UniProtKB:P70096}. Chromosome, centromere {ECO:0000250|UniProtKB:Q99661}. Chromosome, centromere, kinetochore {ECO:0000250|UniProtKB:Q99661}. Note=Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1. Association with microtubule plus ends is also mediated by interaction with KIF18B. Centromeric localization requires the presence of BUB1 and SGO2. {ECO:0000250|UniProtKB:P70096, ECO:0000250|UniProtKB:Q99661}.

Domain: The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends. {ECO:0000250|UniProtKB:Q99661}.

Ptm: Phosphorylation by AURKB, regulates association with centromeres and kinetochores and the microtubule depolymerization activity. {ECO:0000250|UniProtKB:Q99661}.

Ptm: Ubiquitinated. {ECO:0000250|UniProtKB:Q99661}.

Similarity: Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. MCAK/KIF2 subfamily. {ECO:0000255|PROSITE- ProRule:PRU00283}.

Annotations taken from UniProtKB at the EBI.


Organism:		Mus musculus (Mouse).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus.



Function:		In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells (PubMed:14980225). Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis. Plays a role in chromosome congression and is required for the lateral to end-on conversion of the chromosome-microtubule attachment (By similarity). {ECO:0000250\|UniProtKB:Q99661, ECO:0000269\|PubMed:14980225}.


Subunit:		Interacts with CENPH. Interacts with MTUS2/TIP150; the interaction is direct. Interacts with MAPRE1; the interaction is direct, regulated by phosphorylation and is probably required for targeting to growing microtubule plus ends. Interacts with KIF18B at microtubule tips; this interaction increases the affinity of both partners for microtubule plus ends and is required for robust microtubule depolymerization. Phosphorylation by AURKA or AURKB strongly reduces KIF18B-binding. {ECO:0000250\|UniProtKB:Q99661}.
Subcellular location:		Cytoplasm, cytoskeleton {ECO:0000250\|UniProtKB:Q99661}. Nucleus {ECO:0000250\|UniProtKB:P70096}. Chromosome, centromere {ECO:0000250\|UniProtKB:Q99661}. Chromosome, centromere, kinetochore {ECO:0000250\|UniProtKB:Q99661}. Note=Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1. Association with microtubule plus ends is also mediated by interaction with KIF18B. Centromeric localization requires the presence of BUB1 and SGO2. {ECO:0000250\|UniProtKB:P70096, ECO:0000250\|UniProtKB:Q99661}.
Domain:		The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends. {ECO:0000250\|UniProtKB:Q99661}.
Ptm:		Phosphorylation by AURKB, regulates association with centromeres and kinetochores and the microtubule depolymerization activity. {ECO:0000250\|UniProtKB:Q99661}.
Ptm:		Ubiquitinated. {ECO:0000250\|UniProtKB:Q99661}.
Similarity:		Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. MCAK/KIF2 subfamily. {ECO:0000255\|PROSITE- ProRule:PRU00283}.